Pulsed Electromagnetic Field (PEMF) Treatment Reduces Lipopolysaccharide-Induced Septic Shock in Mice
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"In the current study, we examined the effect of PEMF therapy on a mouse model of lipopolysaccharide (LPS)-induced septic shock. Mice injected with LPS and treated with PEMF showed higher survival rates compared with the LPS group. The increased survival was correlated with decreased levels of pro-inflammatory cytokine mRNA expression and lower serum nitric oxide levels and nitric oxide synthase 2 mRNA expression in the liver compared with the LPS group. In the PEMF + LPS group, there was less organ damage in the liver, lungs, spleen, and kidneys compared to the LPS group. To identify potential gene targets of PEMF treatment, microarray analysis was performed, and the results showed that 136 genes were up-regulated, and 267 genes were down-regulated in the PEMF + LPS group compared to the LPS group. These results suggest that PEMF treatment can dramatically decrease septic shock through the reduction of pro-inflammatory cytokine gene expression. In a clinical setting, PEMF may provide a beneficial effect for patients with bacteria-induced sepsis and reduce septic shock-induced mortality."
"The PEMF device used in this study consists of six-channel magnetic flux cores. The dimension of each individual core is 60 mm (outer diameter) by 35 mm (inner diameter) by 30 mm (height). A set of six cores were placed underneath the mouse housing cage and the mice were exposed to PEMF continuously. To determine the extent of the PEMF exposure, we assessed the intensity of the PEMF following the distance from the core. Since a PEMF frequency greater than 75 Hz did not show significant differences in the PEMF intensity indicated in Gauss units, we selected a PEMF frequency of 75 Hz (146.7 Gauss) for subsequent experiments."
"To examine whether PEMF treatment could ameliorate septic shock-induced mortality, mice were injected with a single dose of LPS into the peritoneum and continuously exposed to PEMF for up to 5 days. [...] On the final evaluated day, the mortality was 90% in the LPS group and 54% in PEMF + LPS group (p < 0.001). The sham mice and mice treated with PEMF alone showed no mortality for the duration of the experiment. These results show that PEMF significantly decreased septic shock-induced death in mice compared with LPS-treated mice without PEMF treatment."
"Since the histological analysis of the spleen tissues showed that LPS deteriorated the white pulp structure containing B cells, we wondered whether B cells contributed to the protective effects of the PEMF treatment. To test this hypothesis, B cell deficient μMT mice were injected with LPS (7 mg/kg) and then exposed to PEMF treatment for up to 3 days. Most mice in the PEMF + LPS group and LPS group died within 3 days (Figure 7). There was no statistical significance between the PEMF + LPS and LPS groups. These results suggest that the protective effect of PEMF requires the presence of B cells."
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@stag wtf i love emf now
