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    Nuclear Peating

    Scheduled Pinned Locked Moved Bioenergetics Discussion
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    • alfredoolivasA Offline
      alfredoolivas @alfredoolivas
      last edited by

      And please freeze each load, to preserve your godly neck genetics for humanity.

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      • C Offline
        CrumblingCookie @engineer
        last edited by CrumblingCookie

        @engineer said in Nuclear Peating:

        a phase contrast microscope would let you quantitatively monitor another metabolic factor on the order of every couple days

        These changes however will be a reflection of what influenced metabolism up to 3 months ago. Depending on which phase of development or maturation had been affected. Tracking and evaluation of any influencing factors requires consideration of the delay and a lot of longitudinal data.

        For the quantitative measurements you can probably get an AI programmed to recognize numbers and motility etc. from short video clips. No need to rasterize and manually count. Could be little effort for valuable long-term data.

        hwisdomH 1 Reply Last reply Reply Quote 0
        • engineerE Offline
          engineer @alfredoolivas
          last edited by

          @alfredoolivas said in Nuclear Peating:

          @engineer record the volume of each load you eject too plz

          I did try this recently with a measuring cup and got... 8mL? Apparently this is a lot?

          alfredoolivasA 1 Reply Last reply Reply Quote 3
          • alfredoolivasA Offline
            alfredoolivas @engineer
            last edited by

            @engineer yeah you are shooting ROPES

            1 Reply Last reply Reply Quote 1
            • engineerE Offline
              engineer
              last edited by

              The phase contrast microscope has arrived

              6745.jpg

              It's an old Nikon from possibly the 80s or 90s but is still great!

              alfredoolivasA 1 Reply Last reply Reply Quote 1
              • alfredoolivasA Offline
                alfredoolivas @engineer
                last edited by

                @engineer now for the best part 💦 💦 💦

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                • engineerE Offline
                  engineer
                  last edited by engineer

                  The results are in (translation: there's stuff happening down there)

                  _DSF1541small.JPG

                  It looks slightly terrible on camera for some reason but is much clearer in person. Also, I don't have any proper microscope slides yet so the plastic bag used here is probably making it look slightly worse.

                  Next steps: get special slides and a warmer for them and maybe a better objective lens

                  Edit: looks like I also completely forgot to focus the condenser annulus.

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                  • hwisdomH Offline
                    hwisdom @CrumblingCookie
                    last edited by

                    @CrumblingCookie u still produce sperm daily tho like wut

                    MILF
                    man I love fasting

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                    • engineerE Offline
                      engineer
                      last edited by

                      Just ordered a Nikon Plan 100x/1.25 DL Ph4 objective

                      Stay tuned for more 😉

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                      • engineerE Offline
                        engineer
                        last edited by

                        Update

                        The 100x objective has arrived. Let's do some more Peaty 🍊 metrology 🥕 soon.

                        yerragY 1 Reply Last reply Reply Quote 1
                        • yerragY Offline
                          yerrag @engineer
                          last edited by

                          @engineer
                          Very nice.

                          i may be able to get one setup going myself. Have an old German stereo microscope but lacking eyepieces and a phase contrast attachment. Im just not sure if I should buy them, as they may turn out to be suboar in quality.

                          Its better to get a whole unit like the Nikon that you got.

                          But being to analyze sperm is one additional use for the microscope, which I had originally thought of using for live blood analysis.

                          Temporal thinking is the faculty that’s
                          engaged by an enriched environment, but it’s
                          wrong to call it “thinking,” because it’s simply
                          the way organisms exist... - Ray Peat Nov 2017 Newsletter

                          engineerE 1 Reply Last reply Reply Quote 0
                          • engineerE Offline
                            engineer @yerrag
                            last edited by

                            @yerrag I was under the impression that blood analysis needed specialized lab tests with consumables and not necessarily a microscope. Or are you doing something different?

                            alfredoolivasA 1 Reply Last reply Reply Quote 0
                            • alfredoolivasA Offline
                              alfredoolivas @engineer
                              last edited by

                              @engineer you can test cholesterol, lactate at home etc I think

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