Nuclear Peating
-
The phase contrast microscope has arrived
It's an old Nikon from possibly the 80s or 90s but is still great!
-
@engineer now for the best part
-
The results are in (translation: there's stuff happening down there)
It looks slightly terrible on camera for some reason but is much clearer in person. Also, I don't have any proper microscope slides yet so the plastic bag used here is probably making it look slightly worse.
Next steps: get special slides and a warmer for them and maybe a better objective lens
Edit: looks like I also completely forgot to focus the condenser annulus.
-
@CrumblingCookie u still produce sperm daily tho like wut
-
Just ordered a Nikon Plan 100x/1.25 DL Ph4 objective
Stay tuned for more
-
Update
The 100x objective has arrived. Let's do some more Peaty
metrology 
soon. -
@engineer
Very nice.i may be able to get one setup going myself. Have an old German stereo microscope but lacking eyepieces and a phase contrast attachment. Im just not sure if I should buy them, as they may turn out to be suboar in quality.
Its better to get a whole unit like the Nikon that you got.
But being to analyze sperm is one additional use for the microscope, which I had originally thought of using for live blood analysis.
-
@yerrag I was under the impression that blood analysis needed specialized lab tests with consumables and not necessarily a microscope. Or are you doing something different?
-
@engineer you can test cholesterol, lactate at home etc I think
-
@engineer I had done done blood analysis once with a naturopath/wholistic doctor. He had to spend about one and a half hour to analyze one blood sample as the sample showed some movement that needed time to identify as a fungus coming out of the red blood cell, indicating also there was hemolysis. Though the hemolysis was not of such a large amount that under a urinalysis it would not be detected. But I can see that my urine was amber colored and that visual could mean anything. But after taking turpentine and artemisia annua, which are antifungals, I could see that my urine color had turned to a definite bright yellow that is closer to mello yello or mountain dew, neon yellow even.
Still, that wouldn't even explain why I have a high LDH (250) and a high RDW (13.6), which I would attribute to an ongoing hemolysis of a lower rate of occurrence. Bexause of that hemolysis, destroyed red blood cells would get recycled by the speen and there would be an endogenous source of carbon monoxide produced. This would be confirmed by 2 occurrences of sudden drops in heart rate per minute, transient enough to appear like a stalactite pattern each time in a chart of my heart rate. As the drops should normally be accompanied by drops in spO2 were it a case of methemoglobinemia (oxygen lacking) in an spO2 chart from a ommon pulse oximeter, one would know that if there were no accompanying spO2 drops, it would indicate the drops were caused by the presence of carbon monoxide, as regular oximeters cannot detect CO as distinct from O2. A Massimo co-oximeter would do that, but it is expensive and only trauma centers have that.
I have managed to do with these workarounds, so there is no rush to have a phase contrast microacope. But having a phase contrast microscope around would be helpful as I try to stop the low level (though impactful) of hemolysis. I suspect it is of fungal nature (aspergillus niger) and I think it lowers my metabolism by inhibiting cytoxhrome oxidase, affecting the ETC in mitochondrial respiration. The colony of pathogens also require my innate immune system to onstantly produce inflammatory and oxidative responses that require a corresponding antioxidative response from my body (to prevent tissue destruction) and this depleted my albumin stores in blood. This causes hypovolemia and requires my body to compensate with higher blood pressure. And so far, 25 years since I began fixing my high BP, I am still stuck figuring out how to overcome this wall. But high BP is really hard to cure. Standard medicine only treats it.
Hello! It looks like you're interested in this conversation, but you don't have an account yet.
Getting fed up of having to scroll through the same posts each visit? When you register for an account, you'll always come back to exactly where you were before, and choose to be notified of new replies (either via email, or push notification). You'll also be able to save bookmarks and upvote posts to show your appreciation to other community members.
With your input, this post could be even better 💗
Register Login