Nuclear Peating
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@engineer said in Nuclear Peating:
a phase contrast microscope would let you quantitatively monitor another metabolic factor on the order of every couple days
These changes however will be a reflection of what influenced metabolism up to 3 months ago. Depending on which phase of development or maturation had been affected. Tracking and evaluation of any influencing factors requires consideration of the delay and a lot of longitudinal data.
For the quantitative measurements you can probably get an AI programmed to recognize numbers and motility etc. from short video clips. No need to rasterize and manually count. Could be little effort for valuable long-term data.
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@alfredoolivas said in Nuclear Peating:
@engineer record the volume of each load you eject too plz
I did try this recently with a measuring cup and got... 8mL? Apparently this is a lot?
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@engineer yeah you are shooting ROPES
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The phase contrast microscope has arrived
It's an old Nikon from possibly the 80s or 90s but is still great!
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@engineer now for the best part
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The results are in (translation: there's stuff happening down there)
It looks slightly terrible on camera for some reason but is much clearer in person. Also, I don't have any proper microscope slides yet so the plastic bag used here is probably making it look slightly worse.
Next steps: get special slides and a warmer for them and maybe a better objective lens
Edit: looks like I also completely forgot to focus the condenser annulus.
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@CrumblingCookie u still produce sperm daily tho like wut
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Just ordered a Nikon Plan 100x/1.25 DL Ph4 objective
Stay tuned for more
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Update
The 100x objective has arrived. Let's do some more Peaty
metrology 
soon. -
@engineer
Very nice.i may be able to get one setup going myself. Have an old German stereo microscope but lacking eyepieces and a phase contrast attachment. Im just not sure if I should buy them, as they may turn out to be suboar in quality.
Its better to get a whole unit like the Nikon that you got.
But being to analyze sperm is one additional use for the microscope, which I had originally thought of using for live blood analysis.
