Nuclear Peating

engineer

Just ordered a Nikon Plan 100x/1.25 DL Ph4 objective

Stay tuned for more

engineer

Update

The 100x objective has arrived. Let's do some more Peaty metrology soon.

yerrag

i may be able to get one setup going myself. Have an old German stereo microscope but lacking eyepieces and a phase contrast attachment. Im just not sure if I should buy them, as they may turn out to be suboar in quality.

Its better to get a whole unit like the Nikon that you got.

But being to analyze sperm is one additional use for the microscope, which I had originally thought of using for live blood analysis.

engineer

@yerrag I was under the impression that blood analysis needed specialized lab tests with consumables and not necessarily a microscope. Or are you doing something different?

alfredoolivas

@engineer you can test cholesterol, lactate at home etc I think

yerrag

@engineer I had done done blood analysis once with a naturopath/wholistic doctor. He had to spend about one and a half hour to analyze one blood sample as the sample showed some movement that needed time to identify as a fungus coming out of the red blood cell, indicating also there was hemolysis. Though the hemolysis was not of such a large amount that under a urinalysis it would not be detected. But I can see that my urine was amber colored and that visual could mean anything. But after taking turpentine and artemisia annua, which are antifungals, I could see that my urine color had turned to a definite bright yellow that is closer to mello yello or mountain dew, neon yellow even.

Still, that wouldn't even explain why I have a high LDH (250) and a high RDW (13.6), which I would attribute to an ongoing hemolysis of a lower rate of occurrence. Bexause of that hemolysis, destroyed red blood cells would get recycled by the speen and there would be an endogenous source of carbon monoxide produced. This would be confirmed by 2 occurrences of sudden drops in heart rate per minute, transient enough to appear like a stalactite pattern each time in a chart of my heart rate. As the drops should normally be accompanied by drops in spO2 were it a case of methemoglobinemia (oxygen lacking) in an spO2 chart from a ommon pulse oximeter, one would know that if there were no accompanying spO2 drops, it would indicate the drops were caused by the presence of carbon monoxide, as regular oximeters cannot detect CO as distinct from O2. A Massimo co-oximeter would do that, but it is expensive and only trauma centers have that.

I have managed to do with these workarounds, so there is no rush to have a phase contrast microacope. But having a phase contrast microscope around would be helpful as I try to stop the low level (though impactful) of hemolysis. I suspect it is of fungal nature (aspergillus niger) and I think it lowers my metabolism by inhibiting cytoxhrome oxidase, affecting the ETC in mitochondrial respiration. The colony of pathogens also require my innate immune system to onstantly produce inflammatory and oxidative responses that require a corresponding antioxidative response from my body (to prevent tissue destruction) and this depleted my albumin stores in blood. This causes hypovolemia and requires my body to compensate with higher blood pressure. And so far, 25 years since I began fixing my high BP, I am still stuck figuring out how to overcome this wall. But high BP is really hard to cure. Standard medicine only treats it.

engineer

@alfredoolivas looks like you can also test red blood cell count.

https://microscopeinternational.com/dark-line-etched-hemacytometer/

This same site sells a bunch of these counting slides so it's interesting how many tests are possible with a microscope.

engineer

Bad news: A reusable counting chamber for Nuclear Peating is another $1000.

https://microscopeinternational.com/makler-counting-chamber/

engineer

@yerrag I didn't realize such a fungal infection could affect your blood pressure in that way. In that case, I believe Ray Peat had ideas to cure fungal invasions so I'm wondering if you've considered those or not. Something I would personally try is a megadose of magnesium as acute amounts drastically lower BP, enough for use in hospitals. That's actually how my mom didn't die during my birth, a huge magnesium IV.

engineer

Update

I've reluctantly ponied up the extra grand for the high precision grid counter slide so that we can finally get some hard numbers.