Posts made by ursidae

@ThinPicking
Yeah I'm thinking acidic water with some phosphoric acid, NaH2PO4 and a good amount of fructose. HFCS is notorious for this. Maybe some tryptophan, methionine, cysteine too. Gonna have to look up solubility

by only using water soluble compounds

@ursidae

nevermind, apparently that's not okay to do either. Can't do anything except add lab salts to the water

@Amazoniac
thank you
I was told I can only use the stuff in the cupboard unfortunately and nothing like tartaric acid can be ordered

I've been thinking of injecting them with bicarbonate twice a week or more. I'll bypass the gut and there will be no more acid conundrums. Gonna be doing a literature search on the methods and moles of how this has been done.

3 groups for now. Sadly I only got 10 animals. 4 animals tap water, 2 of them injected with bicarbonate
3 animals with tap water diluted with deionised to reduce the buffering capacity/ionic strength, 1 of them injected with bicarbonate
3 animals with tap water enriched with MgSO4, KH2PO4 and NaHCO3, 1 injected with bicarbonate

@Amazoniac

thank you so much

I'm not sure if the guys at the lab will provide me with tartaric acid. They're generally against using organic acids for this experiment.

I'm trying to find some facts about its pharmacokinetics, where do you source your information ?

If it's indeed inert and wouldn't affect the micro biome too drastically then I'll use it

I want to try and maximise the difference between the two waters by mimicking the natural variation among tap/spring/mineral
waters

Silicilic acid sounds like a good idea to me actually
There's brands with 60 mg/L of it here

then there's some waters that are naturally at pH 4.5 due to some amount of phosphoric acid

If I use a mix of silicilic and phosphoric with some of their conjugate salts (and possibly tartaric) to bring the pH down and then perhaps a little bit of HCl then the mice wouldn't be ingesting too many chloride ions. I could even use hypochlorous acid instead of HCl because it's a weak acid

water acidified with HCl causes acid base imbalance, I think acidosis due to H3O+ excess plus bicarbonate depletion due to the chloride overload (hyperchloremic acidosis)

I can't use citric (my first choice) or acetic or any organic acid that interferes with glucose metabolism for this

I can't add carbonate salts and then HCL because they get hydrated to carbonic acid at pH under 4 and the equilibrium is shifted to the left and it cycles between CO2 and H2CO3, leaving the system little by little in the form of CO2

if I carbonated the water after modification with HCL and NaHCO3, the CO2 would leave the bottle quickly because the hydronium ions favour the left side and theoretically CO2 would build up in the air in the bottle, shifting the equilibrium to the right but it would only enter the system at the points where the air is in contact with water which is still almost nothing

no possibility for high temperature or high pressure storage of the water

also needs to be autoclaved which would cause explosions if carbonated

Could I bring water to pH 5 with HCL then turn it into a phosphate buffer with phosphoric acid plus calcium dihydrophosphate plus calcium chloride (to get a healthy P: Ca ratio ? Then add potassium bicarbonate and it cycles between the salt and carbonic acid form because it's a buffer rather than leaving as CO2
how would the phosphoric harm the organism? It's a product of protein metabolism along with sulfuric so it would probably also cause acidosis just like HCL

looks like I had the right hunch

the woman's PTH is double the reference range

high tCO2 in blood, high SB, ionised calcium in the upper range, normal vitamin D

Awesome guys

thank you. I'll now be reading all this. I already read the first link of the Peat article and I think the PTH angle is especially relevant for the particular person who is complaining of high BP

@Amazoniac

hey man

Please share everything you know about high (and low) blood pressure