Bile can serve as a reservoir for funghi, making them harder to treat

[yerrag]

@Mauritio

Glad that it worked for you the 2nd time around. It could be about cycling, as that is something I forget to do at times. It it could be that you changed that made your interior more conducive to being fixed.

[yerrag]

@dapose You hit many right notes there. Usually there is an imbalance and that leads to a dominance that is seen as pathogenic. Perhaps we should see restoring balance as the goal. We never really kill or eliminate like genocides are natural, we only discourage dominance because uneven playing fields make it happen. Intentional or nor.

[CrumblingCookie]

@Mauritio said:

we need small and hydrophilic molecules for an anti-fungal effect

Im looking for anti-fungal candidates that fit the above category:

Encapsulated calciumhypochlorite granules ("Chloryte") may fit this bill for the upper GI and various deep tissues. It's rather rough and destructive and not a thing to do for more than two weeks at the very most, IMO.

@Mauritio said:

I took Haarlem oil daily for a while.
I believe it contains turpentine and sulfur. I got less and less of an effect as time went on so I stopped. But I recently reintroduced it and found that cycling it, seems to maintain its effects.

Turpentine (or more generally monoterpenes) reduce hepatic cholesterol synthesis, thereby reducing the cholesterol-saturation of bile which is then able to gradually dissolve cholesterol "plaque" or even proper stones along the bile ducts and in the gall bladder. That improves the biliary milieu along with better biliary flow. It's a very long-term process, however. It takes several months of taking monoterpenes ("Rawachol" capsules or tincture is an OTC product) 2-3 times daily. Tudca or Udca works well together with monoterpenes.

@Mauritio said:

I'm not sure how the treatment should look.
Increasing transit speed and frequently emptying the gallbladder by eating fiber, using specific supplements like FXR-agonists

Tudca and Udca also reduce the same enzyme of hepatic cholesterol synthesis, while additionally altering the bile acids composition towards being much more hydrophilic and less sticky, and also increasing the bile salt export pump for an in overall much greater bile flow (less stasis).
I'd rather use those daily instead of doing any more gall bladder flushes ever again (which only work in a very mechanical and macroscopic way).

[dapose]

@yerrag
Well said Ye!
I would say heavy hitters in the militaristic approach to a balanced system would be Castor Oil packs on the skin over the liver.
Higher dose Aloe Vera Juice.
Oregano oil + activated charcoal.
Russian Sauna. Or fever.
And Swedish Bitters one shot in warm chamomile tea.
The big guns!
Have you ever tried clove tea for your lungs!!?? Supposed to be great!!!

[Mauritio]

@Sunniva said in Bile can serve as a reservoir for funghi, making them harder to treat:

(DMSO) inhibits the germination of Candida albicans

That is so interesting . You just helped me to connect the dots.
Several times when I used Melanon topically on the area above my liver/gallbladder I experienced fungal die off. I attributed it to apigenin or naringenin, but it might as well be the DMSO.
Apigenin and naringenib are also anti-fungal. They're also small molecules, but not hydrophilic. But still Melanon should be a very effective anti fungal applied topically.
https://pubmed.ncbi.nlm.nih.gov/29346565/
https://pubmed.ncbi.nlm.nih.gov/33934490/

If the gallbladder is so hard to reach for anti-fungals maybe topical is the way to go.
Maybe using some anti-fungal IdeaLabs supplements over the gallbladder would be good, ideally one that uses DMSO as a solvent.

Alternatively dissolving MSM in DMSO should help. It is a small, hydrophobic molecule and it dissolves in MSM so it might be very potent if delivered directly to the gallbladder via topical application.

[CrumblingCookie]

@Mauritio
Very nasty! Thanks for pointing me back to this thread.
One thing I was immediately wondering about is whether the less-lipophilic fluconazole would then be more potent against bile fungi than the significantly more lipophilic itraconazole, even though fluco is quite completely being excreted renally and itra biliary.
But the answer lay in your linked study: Fluco was shown to be useless in bile. Utterly.
There no mentioning of itra in this context. Only a remark, that fungi increase their itraconazole-resistance in the presence of cholesterol. However, this mechanisms affects all azoles. Essentially, candida and other fungi can salvage exogenous cholesterol to make up for loss of ergosterol synthesis and are thus less affected by the inhibiting impact of azoles.

So how to tackle these biliarly persisters?
I doubt they will only serve as a reservoir without already being harmful where they are. In your linked studies it says, C. albicans hyphenates upon contact with bile acids. I therefore assume they do bad things already in the liver and gallbladder and potentially alter the function of these organs or of the bile composition. @bearwithme

The water-soluble flucytosine is available as 500mg tablets (ANCOTIL) on prescription for specific systemic fungal infections... certainly nothing anyone would easily obtain officially from a doctor because of a hunch of biliary fungal persistence!
I had never heard of it before.
Side effects are kind of the usual; don't get pregnant or impregnate anyone else within 6 months, blood cell discorders, skin detachment, fatal hepatic lesions, hypokalemia and heart troubles, the whole cluster of confusion, headaches, vertigo, neuropathies, hallucinations as well as respiratory impairments or arrest and reduced kidney functions.
What do you peeps reckon - is it wortwhile to try out?

@Mauritio said in Bile can serve as a reservoir for funghi, making them harder to treat:

Im looking for anti-fungal candidates that fit the above category:

MSM
Small molecule and highly water soluble , fits the bill.

Any info on how methylsulfonylmethane would be antifungal?

Oregano oil or winter savory oil mostly for the carvacrol and thymol are again strongly lipophilic.

Berberine is strongly synergistic with azoles and can overcome azole-resistance.
Propolis extract is antifungal as well and can overcome azole-resistance.
Boron.
Balsamic turpentine may be mostly anti-biofilm.
Especially boron may be mostly preventative at regular low doses but tbh I'm really disappointed with all of these on their own not being anywhere near to being effective on their own.

Maybe a topical antifungal like nystatin or amphotericin B for the GI lumen over quite a long term, plus a combination of systemic substances: boron, berberine, propolis extract, + pharmaceutical antifungal?

[CrumblingCookie]

@Mauritio said in Boron supplements:

@CrumblingCookie I agree with you on subclinical fungal infections.
They're very hard to get rid of. I posted a study about fungi hiding in the gallbladder when attacked. Let that sink in.

It's just as bad when we look into CNS persistence of fungal infections in chronic brain disorders, Alzheimers and dementia:
Tulane University & Tulane National primate research center, 2022

The potential contribution of pathogenic microbes to dementia-inducing disease is a subject of considerable importance. Alzheimer’s disease (AD) is a neurocognitive disease that slowly destroys brain function, leading to cognitive decline and behavioral and psychiatric disorders. The histopathology of AD is associated with neuronal loss and progressive synaptic dysfunction, accompanied by the deposition of amyloid-β (Aβ) peptide in the form of parenchymal plaques and abnormal aggregated tau protein in the form of neurofibrillary tangles.

Observational, epidemiological, experimental, and pathological studies have generated evidence for the complexity and possible polymicrobial causality in dementia-inducing diseases. The AD pathogen hypothesis states that pathogens and microbes act as triggers, interacting with genetic factors to initiate the accumulation of Aβ, hyperphosphorylated tau protein (p-tau), and inflammation in the brain. Evidence indicates that Borrelia sp., HSV-1, VZV (HHV-2), HHV-6/7, oral pathogens, Chlamydophila pneumoniae, and Candida albicans can infect the central nervous system (CNS), evade the immune system, and consequently prevail in the AD brain. Researchers have made significant progress in understanding the multifactorial and overlapping factors that are thought to take part in the etiopathogenesis of dementia; however , the cause of AD remains unclear .

“It has been suggested that Aβ functions as an antimicrobial peptide. Interestingly, C. albicans was found to be sensitive to synthetic Aβ and brain homogenates from AD patients that were capable of inhibiting fungal growth [178]. Additionally, it was demonstrated that Aβ protects against C. albicans in glial cells as well as invivo in nematodes [179]. ”

“Alonso and colleagues provided extensive evidence that disseminated mycoses are potential causative agents or risk factors for AD [173,180]. Different fungal genera detected in AD brain tissue include Malassezia, Fusarium, Candida, Cladosporium, Alternaria, and Botrytis [181]. An analysis of CSF revealed significant levels of Candida albicans and Candida glabrata in samples from AD patients. Approximately >89.6% of serum from AD patients tested positive for antibodies to Candida compared to 8.8% for controls [173].”

“The different species that were detected included Saccharomyces cerevisiae, Malassezia globosa, Malassezia restricta, and Penicillium. Furthermore, this group detected yeast and fungal proteins, including (1,3)-β-glucan, fungal polysaccharides, and mycoses, in the peripheral blood of AD patients, which suggests that a chronic fungal infection may increase the risk of dementia [173,180]. More strikingly, yeast-like cells and hyphal structures were observed in CNS tissue from AD patients using polyclonal antibodies against a variety of fungi [181].

Pisa et al. also provided strong evidence for fungal infection in AD patients [170]. Brain sections derived from AD patients showed that all were infected with fungi [170]. Fungal material was detected intra- and extracellularly in the neurons of tissues of AD patients, but no fungal material was observed in tissue from control individuals [170]. Moreover , fungal DNA and proteins were also found in brain regions including the frontal cortex, hippocampus, cerebellar hemisphere, and choroid plexus but not in control patient tissue. ”

[sunsunsun]

idk about in context of bile, but iron chelation in fungi via doxycycline makes fluconazole anti fungal against some candida rather than just fungistatic or no effect. aspirin is also an iron chelator.

there’s a study that suggests organ cancers often come with fungal infection of that organ too.

fwiw the warnings about ketoconazole being particularly liver toxic are apparently not actually proven. the symptomatic liver damage incidence rate in all the azoles is about the same.

overall for fungal issues i think the patient gets treatment with a normal pharmaceutical antifungal dose and length (could be months), and then keep the antifungals on hand to pulse dose as needed if symptoms return. and then use things like aspirin and doxycycine, include some niacinamide, sunbathing and nutrition etc to keep the stuff in check. total eradication of fungi seems impossible on a realistic sense

if bile is a reservoir for fungi maybe just eating high bile binding foods regularly can flush it out. rather than recirculating bile more

[sunsunsun]

@CrumblingCookie nystatin is pretty weak ime but i guess it depends on which fungi
ketoconazole cream works way better ime but idk what strains it is working agaisnt. i think mallessezia

berberine seems interesting, is it peaty ? it isnt right, because it is like metformin and metformin isnt peaty

[CrumblingCookie]

Thanks for sharing.
Indeed I currently find myself in a situation wherein I don't know for how long in total to continue fluconazole. Months? It costs about $60/week for me. And it's difficult to say which depressive mental effects are true sides and which are due to various degrees of die-off.
I wonder what role the continous re-infection from bile plays in commonly required treatment length. The notion of it being impossible to clear from tissues is what I find unsettling, too.
Have started to add in berberine today, 500mg thrice daily. Along with ample propolis extract twice daily.
Luckily I've failed to make it to the store in time and stumbled upon some garlic in my kitchen drawers and have read up on allicin again.
One half to a full garlic bulb per day is allegedly a good guideline for powerful effects (about 1mg allicin per kg BW from 3-5mg allicin per g fresh garlic). Ground it/crush it, let it rest for 10 minutes for the allicidase to do its work before diluting it in food or adding any acids like vinegar.

@sunsunsun said in Bile can serve as a reservoir for funghi, making them harder to treat:

iron chelation in fungi via doxycycline makes fluconazole anti fungal against some candida rather than just fungistatic or no effect

That study is in-vitro and super misleading however, because few antibitiocs, if any other at all, are as fungal-infection promoting as the tetracyclines because of their powerful, continuous inhibition of phagocytosis lasting for days even after stopping further intake. Tetracyclines are one of the main risk factors for fungal infections in literature. Ime they are very overhyped and terrible.

As for ketoconazole I'm wary of its unique VDR antagonism which essentially inhibits innate immunity and reduces xenoautophagy of intracellular endomyceles. That also essentially makes ketoconazole topical creams a powerful antiinflammatory for skin conditions, saving the extra prednisolone so enamored by dermatologists.

[sunsunsun]

@CrumblingCookie https://www.sciencedirect.com/science/article/abs/pii/S2213716517302229

[CrumblingCookie]

@sunsunsun Wax moths? They don't even have neutrophils and I won't deep-dive into finding out how their hemolymphs specifically compare to human or mammalian neutrophils.
I really do harbor strong apprehensions against the potential (mis)use of doxy on fungal infections.

What I would much rather do is oral terbinafin + fluconazole.
The terbinafin by itself is useless against many fungi species but by disturbing an earlier point (Erg1) in the sterol biosynthesis pathway it is synergistic with azoles (which act upon Erg11).
allegedly it strongly inhibits expression of the fungal efflux pumps responsible for azole resistance!
(! Berberine is the one which inhibits efflux pump expression. I had mixed it up).
This still won't tackle activitiy in the biliary tract, though.

I've done some more reading of publications on biliary / gallbladder candida / fungi.
Fungi can be causes of calculous (lithiasic) or also acalculous cholecystitis with or without mixed bacterial infections (Escherichia coli, Enterococcus, Klebsiella, Enterobacter, Clostridia).
Even in such rare and often fatal cases that happened to be documented, candidemia was rare despite frequent (almost always) fungal infection of other organs.

https://pubmed.ncbi.nlm.nih.gov/8862389/
https://pubmed.ncbi.nlm.nih.gov/28703118/
https://pmc.ncbi.nlm.nih.gov/articles/PMC8764969/
https://pmc.ncbi.nlm.nih.gov/articles/PMC12338181/
https://pubmed.ncbi.nlm.nih.gov/24474028/
https://pubmed.ncbi.nlm.nih.gov/8207288/

I don't have visible gallbladder wall thickening or distention or an obviously, universally accepted liver disease (except for hepatomegaly) but if I had I sure as hell would want to insist on flucytosine, or flucytosine + i.v amphotericin B along with any broad-spectrum antibiotics and before mutilating surgeries they'd offer.

Have looked more into berberine again, too. It's impossible to achieve its antimicrobial concentrations (at least 0.1mg/mL) systemically. Serum concentrations are about 0.5 - 16 ng/mL. I.e. 0.000005 mg/mL. Ridiculous!
The berberine in practical doses of 500-1500mg will thus be acting mainly within the GI lumen in support of oral Ampho-B. Berberine has low absorption but some will spill over to reach the liver for extensive first-pass metabolism, yet probably not into biliary ducts.

[Mauritio]

Great points, guys.

My current working hypothesis is rotating and combining several anti fungals daily + keeping bile flowing.

If i don't rotate the antifungals they seem to loose effect surprisingly quickly.
But what is obvious from many studies, is that combining certain anti fungals can drastically increase their effectiveness. Nystatin is synergistic with thymoquinone and also thymol IIRC.

The other important part is to keep bile flowing and excreting it. The lowtoxin crowd actually has a point there. Inducing a bile dump seems very therapeutic to me and followed by certain time of remission/ relieve.
So having adequate fiber intake seems important. Pectin is helpful, vitamin D and K as well.
I also ordered an herb that's used for cholestasis in TCM called Artemisia capillaris it has a lot of studies on liver health and general anti-inflammatory effects.

[Mauritio]

@sunsunsun said in Bile can serve as a reservoir for funghi, making them harder to treat:

idk about in context of bile, but iron chelation in fungi via doxycycline makes fluconazole anti fungal against some candida rather than just fungistatic or no effect. aspirin is also an iron chelator.

Makes sense. I've been using an iron cast pan for years and my ferritin came back very high.
Maybe that contributed to my susceptibility to (fungal) infections.

@sunsunsun said in Bile can serve as a reservoir for funghi, making them harder to treat:

total eradication of fungi seems impossible on a realistic sense

if bile is a reservoir for fungi maybe just eating high bile binding foods regularly can flush it out. rather than recirculating bile more

Yes agreed

[Mauritio]

@CrumblingCookie said in Bile can serve as a reservoir for funghi, making them harder to treat:

Any info on how methylsulfonylmethane would be antifungal?

They say it's the sulfur in it. There's not a lot of evidence for that though. From my experience it is definitely anti-fungal.
I suspect it can chelate metals. Usually that doesn't just involve the bad heavy metals but also the good ones. So Id make sure to test my minerals and keep intake high when taking it long term.

[yerrag]

@Mauritio

For what it's worth, let me throw in some tidbits from what I've conjectured over my 2 decades of trying to lower my blood pressure. I've done a lot from eliminating a lot of periodontal bacteria that has translocated to my vascularure to chelating lead lately using Emeramide. Through a process of elimination of these possible causes, I have been halfway successful only though in lowering my blood pressure. From 260/160 to a current 169/110, I still have ways to go.

But I still some light at the end of the tunnel though. And it is something I have overlooked. As fungal infection has always been under the radar and an afterthought inmedical circles. Doxtors would treat bacteria with antibiotics, and rarely talk about fungus. A confinement in a hospital would see a patient get well from antiiotic administration, but a discharge would be delayed only because the good doctor for some hunch decides to give some antifungals..

I would later realize that some antibiotics are known to transform bacteria into cell wall- deficient microbes that are harder to kill because they lack this cell wall which is used to kill microbes with antibiotics. This transformation from bacterial form to a form halfway between bacteria and a fungi ia at the hear of a ' competing medical dogma called terrain theory, which is opposed to the mainstream model based on herm theory- where all microbes are accepted as being static in nature, and do not pleomorph, as would be what happens when commensal and regulatory (aka friendly bacteria) turn from Dr. Jekyll into Dr. Hyde, and become pathogenic.

But this should explain to me why it has become common practice for doctors to administer antifungal drugs after successfully treating a patient with antibiotics. But they cannot explain why, for to say the real reason is to somehow agree that terrain theory is real, which is anathema to their cornerstone germ theory.

But back to my vexing problem of fixing my high BP problem, which is on its 26th year.

Lately, I had began taking artemisia annua tincture which saw me successfully lower my BP from 200./150 to 150/100. I took this as an antifungal to hyphal aspergillosis (aspergillus niger is also known as black mold. How I got this is another story).

It would be nice if my BP kept going down to 120/80. That would be a nice end to this story.

But my BP held at around 150/100 - 160/110. I was still urinating foamy urine. And I realize that this is the result of a continuing activation of neutrophils and macrophages th as t I would conclude from my CBC test, showing my wbc to be high and my neutrophils and monocytes to be high.
Even as my eosinophils have gone down as the large hyphal aspergillus fungal parasite colony have been markedly reduced. But the fungus took on a smaller form called a clamydophore. And in this form, more neutrophils and macrophages were needed to kill or neutralize them.
This is the reason why in my CBC test, there would be a high count both in neutrophils and monoxytes.

Yet Iinflammation and oxidative stresses from ROS being produced and spilling over to destroy adjacent tissues would keep occurring, and that my body would have to use its antioxidants to protect tissues from.being destroyed. The main extracellular antioxidant would be called upon again, and albumin would be oxidized and excreted in urine. Which would explain foam in my urine. As well as mynhigh BP as my albumin stores in plasma would be insufficient to hold on to salt, with salt being needed to keep my plasma and blood volume high enough. The body compensates by increasing blood pressure.

But this fungal presence is hard to destroy. As you would concur. What I am trying now, in addition to turpentine and artemisia annua, is the use of Lugol's Iodine. In the next weeks and months, I will find out.

Looking back, I can't help but see why my LDH has always been high at around 260. I think it is because I have hemolysis. Under the radar. It is from an underlying fungal presence that needs to feed off iron from red blood cells it breaks down.

Since heme oxygenase enzyme breaks down the heme from hemolyzed red blood cells, the carbon monoxide could also explain why when I look at my heart rate chart, I would see occasional transient sharp drop in my heart rate even when my spO2 chart says I have a lot of oxygen at that given time. It is because a typical.oximeter cannot distinguish carbon monoxide from.oxygen, and the carbon monoxide is masking as oxygen, even as my heart cannot use the carbon monoxide to produce energy. I have a bit of carboxyhemoglobinemia. That is how I see it. And that would be a result if an ongoing case of chronic low level hemolysis caused by iron-hungry aspergillus fungi. The same fungi that is causing high BP stealthily.

Fungi remains as a. bogeyman to us.