@Mulloch94 Perhaps you miss a fundamental step that by the time the substance leaves the system the final acetylcholine will perhaps be higher than before. Do you understand what I mean? a typical example is when you use Cypro for example you might feel terrible during it but when it comes out you will feel better due to a matter of receptor under-regulation and over-regulation. I gave a random example but it could apply to anything we introduce. I absolutely need to reactivate acetylcholinesterase or antagonize whatever is blocking the enzyme. They mention atropine scopolamine but the hospital should do it, not me!

Glycerophosphocholine (GPC) is the primary form of choline that occurs in milk. It's purposed as a cognitive enhancer, but the molecule yields different products after metabolism, with a distribution beyond the brain.

The dose used below, extrapolated to humans, would be something like 1 g/70 kg, which isn't too crazy:

Choline | WebMD

"In supplements, choline has most often been used by adults in doses of 1-3 grams by mouth daily for up to 4 months."

Absorption, tissue distribution and excretion of radiolabelled compounds in rats after administration of [14C]-L-a-glycerylphosphorylcholine

"aGPC was labelled with [14C]-glycerol ([14G]-GPC) or [14C]-choline ([14C]-GPC)."

"Both labelled compounds gave a wide distribution of radioactivity, particularly concentrated in the liver, kidney, lung and spleen compared to blood."

adde4268-3bc6-4570-b7be-ce48814af0b9-image.png

"After oral administration, the main circulating metabolite was choline (after [14C]-GPC); intact a-GPC was not present after either labelled compound although there were the same unknown metabolites as after i.v. injection."

96259bc0-10d1-4e8d-8230-9f5e14ad0b7d-image.png

"[..]choline and/or its anabolites enter the brain and is reused for biosynthesis of phospholipids. This was confirmed by chromatography of the phosphatidylcholine fraction of the brain homogenates after hydrolysis with phospholipase C. As in blood, this hydrolysis resulted in formation of choline phosphate."

"By far, the largest part of the administered radioactivity was exhaled as 14CO2, in accordance with established catabolic pathways of glycerol (i.e. glycolysis) and choline degradation."

0a1a73cd-f7ef-4870-bf07-6f8ac56170ec-image.png