Cysteine depletion triggers adipose tissue thermogenesis and weight loss
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I am wondering if this is part of the reason that the sugar diet works for some people. This was a mouse study.
Cysteine depletion triggers adipose tissue thermogenesis and weight loss (2025)
Caloric restriction and methionine restriction-driven enhanced lifespan and healthspan induces ‘browning’ of white adipose tissue, a metabolic response that increases heat production to defend core body temperature. However, how specifc dietary amino acids control adipose thermogenesis is unknown. Here, we identifed that weight loss induced by caloric restriction in humans reduces thiol-containing sulfur amino acid cysteine in white adipose tissue. Systemic cysteine depletion in mice causes lethal weight loss with increased fat utilization and browning of adipocytes that is rescued upon restoration of cysteine in diet. Mechanistically, cysteine-restriction-induced adipose browning and weight loss requires sympathetic nervous system-derived noradrenaline signalling via β3-adrenergic-receptors that is independent of FGF21 and UCP1. In obese mice, cysteine deprivation induced rapid adipose browning, increased energy expenditure leading to 30% weight loss and reversed metabolic infammation. These fndings establish that cysteine is essential for organismal metabolism as removal of cysteine in the host triggers adipose browning and rapid weight loss.
““Effective health care depends on self-care” - Ivan Illich, 👀
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@DavidPS i posted a human study before that showed cysteine restriction also works in humans .
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@Mauritio And have you tried CD at all, and what results
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Remember that in models where only cysteine is restricted, they are cystathionine γ-lyase or cystathionine β-synthase (CBS) knockout models. In a normal situation if you want to emulate these studies you have to restrict methionine and cysteine, because methionine can be converted into cysteine.
A cysteine-free (no-Cys) diet induced weight loss exclusively in Cse−/− mice, indicating that depletion of newly absorbed and synthesized cysteine is necessary for the effect
Weight loss was completely prevented by supplying cysteine through either N-acetylcysteine (NAC) or GSH (which is broken down to cysteine in the gut)
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Anyone who’s replicated this dietarily, what do you eat?
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does gelatin supplementation emulate cysteine/methionine/tryptophan etc depletion?
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@sunsunsun partly. It enhances it.
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@sunsunsun said in Cysteine depletion triggers adipose tissue thermogenesis and weight loss:
does gelatin supplementation emulate cysteine/methionine/tryptophan etc depletion?
From cronometer.com, here is the amino acid profile of gelatin.
Dr. Peat spoke about the benefits of gelatin beginning at about 16 minutes. He mentions that little old ladies could live off of gelatin and toast. LOL
““Effective health care depends on self-care” - Ivan Illich, 👀
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@DavidPS said in Cysteine depletion triggers adipose tissue thermogenesis and weight loss:
Dr. Peat spoke about the benefits of gelatin beginning at about 16 minutes. He mentions that little old ladies could live off of gelatin and toast. LOL
lol, made me chuckle too
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So, to replicate this, do we just eat gelatin and keto acid sources for protein?
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@LetTheRedeemed - As stated in the opening post:
Systemic cysteine depletion in mice causes lethal weight loss with increased fat utilization and browning of adipocytes that is rescued upon restoration of cysteine in diet.
Cysteine is a conditionally essential amino acid. The US RDI is 287 mg and I would not try to go lower than that amount.
It is very easy to get to the RDI for cysteine. The flipside is that it difficult to keep your levels below the RDI; but it can be done if you have your heart set on it.
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Using gelatin to maintain protein levels during cysteine depletion could cause an imbalance in the Lysine and Arginine ratio in the diet.
From cronometer.com, here is the amino acid profile of gelatin.
It is desirable to have diet's overall ratio of Lysine to Arginine to be more than 1.0 to prevent viral outbreaks. Foods with a higher lysine content compared to arginine are generally recommended to help manage symptoms. I usually have a high amount to dairy in my diet (see chart below). You can also use Lysine supplements to change the overall ratio in your diet.
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What's a quick recipe for easy daily gelatin consumption? Just making fruit jelly?
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@Milk-Destroyer dissolve it in coffee or milk. You can dissolve loads and it’s still easy to drink these drinks. Just heat up a cup of coffee / milk until it’s boiling in the microwave, add in your gelatin, mix and boom.
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So far this diet suffers the same paradox i’ve found before: a food will be low cystine, but high methionine or tryptophan, and vice versa.
Studies have also shown that methionine is a component similar to cystine. Tryptophan can be preferentially converted to serotonin under stress, but in an ideal state uses b2/b6 and iron cofactors to produce niacin and niacinamide…
Besides the gelatin, it Looks like the best food compromise for protein is oatbran. I could try this…. Maybe supplement b2 & b6 and keep a weekly serving of liver/oysters for the iron.
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@alfredoolivas please stop posting disinformation
. gelatin blooms in cold liquid and then is to be heated. putting it in hot liquid directly makes it into concrete.hydrolyzed collagen can be dissolved in whatever, thoughever
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@sunsunsun Have you tried drinking the coffee with the gelatin dissolved in it, whilst it’s still hot ?
That way it won’t turn into concrete.
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I just eat a small spoonful of gelatine straight from the spoon. My beef gelatine tastes wonderful.
But make sure to drink a little water first, so your mouth is moist. Otherwise, you'll definitely need some water afterwards.
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@Ena this feels like a classic troll but you sound too sincere lol…
I could never imagine eating beef gelatin by the spoonful hahah -
@Ena it will have a harder time absorbing if you didn’t dissolve it
