Horseradish

Mauritio

I've been suspecting that horseradish has a nice effect on my mood and hair.
After reading this thread (https://raypeatforum.com/community/threads/horseradish-inactivates-bacterial-endotoxin.17437/ )
it seems to have to do with its ability to bind and deactivate endotoxin.

For me I've long suspected that hair loss is caused or at least greatly affected by a chronic sinus infection. Horseradish obviously travels into the sinuses and can have an anti-bacterial /Anti-endotoxin effect there.

I have to experiment with it more, but these studies already look promising:

Reduces NO,NO synthase, TNFa, IL6 and COX2

"Our results demonstrate that Armoracia rusticana reduced nitric oxide, tumor necrosis factor-α and interleukin-6 release and nitric oxide synthase and cyclooxygenase-2 expression in macrophages, acting on nuclear transcription factor NF-κB p65 activation."
https://pubmed.ncbi.nlm.nih.gov/26411988/

In this study the showed that molecules extracted from horseradish have an antibacterial effect on 6 different oral pathogenic bacteria and also on Candida albicans, which explains why some people report a whiter tongue when eating horseradish regularly.
https://pubmed.ncbi.nlm.nih.gov/24077540/
It seems like horseradish peroxide, which is extracted from horseradish, is very effective at inactivating estrogens from water. Since we probably all get our fair share of estrogens in water or food this might be useful , resulting in an anti-estrogen effect.
https://pubmed.ncbi.nlm.nih.gov/17498772/
https://pubmed.ncbi.nlm.nih.gov/17897698/
If I'm reading that right horseradish should have a pro-thyroid effect. Horseradish peroxide when reacting with water forms molecules that help with thyroid hormone synthesis and conversion.

"As previously shown for the lactoperoxidase FeIV Ro compound, the CcP FeIV Ro compound was involved in the coupling and not in the iodination reactions. In contrast, the HRP FeIV o pi + compound catalyzed both iodination and hormone formation. "
https://pubmed.ncbi.nlm.nih.gov/2982316/

Horseradish peroxide (together with thyroid hormone) oxidizes NADH Back into NAD.
https://pubmed.ncbi.nlm.nih.gov/192281/

Ismail

@Mauritio very interesting! I guess you know what I’ll be trying later

How are you eating the horse radish?

Mauritio

@Ismail said in Horseradish:

@Mauritio very interesting! I guess you know what I’ll be trying later

How are you eating the horse radish?

It's part of a cheese I'm eating. So I discovered it on accident. And that means pretty small amounts are sufficient for me .

alex155

@Mauritio said in Horseradish:

If I'm reading that right horseradish should have a pro-thyroid effect. Horseradish peroxide when reacting with water forms molecules that help with thyroid hormone synthesis and conversion.

"As previously shown for the lactoperoxidase FeIV Ro compound, the CcP FeIV Ro compound was involved in the coupling and not in the iodination reactions. In contrast, the HRP FeIV o pi + compound catalyzed both iodination and hormone formation. "
https://pubmed.ncbi.nlm.nih.gov/2982316/

Horseradish peroxide (together with thyroid hormone) oxidizes NADH Back into NAD.
https://pubmed.ncbi.nlm.nih.gov/192281/

So this means that not all cruciferous plants have a goitrogenic effect?

Mauritio

@alex155 probably depends on the dosage.

tubert

@Mauritio It is anti thyroid though

Peatful

@alex155 said in Horseradish:

@Mauritio said in Horseradish:

If I'm reading that right horseradish should have a pro-thyroid effect. Horseradish peroxide when reacting with water forms molecules that help with thyroid hormone synthesis and conversion.

"As previously shown for the lactoperoxidase FeIV Ro compound, the CcP FeIV Ro compound was involved in the coupling and not in the iodination reactions. In contrast, the HRP FeIV o pi + compound catalyzed both iodination and hormone formation. "
https://pubmed.ncbi.nlm.nih.gov/2982316/

Horseradish peroxide (together with thyroid hormone) oxidizes NADH Back into NAD.
https://pubmed.ncbi.nlm.nih.gov/192281/

So this means that not all cruciferous plants have a goitrogenic effect?

It’s a root
So that is relevant

alex155

@Peatful said in Horseradish:

@alex155 said in Horseradish:

@Mauritio said in Horseradish:

If I'm reading that right horseradish should have a pro-thyroid effect. Horseradish peroxide when reacting with water forms molecules that help with thyroid hormone synthesis and conversion.

"As previously shown for the lactoperoxidase FeIV Ro compound, the CcP FeIV Ro compound was involved in the coupling and not in the iodination reactions. In contrast, the HRP FeIV o pi + compound catalyzed both iodination and hormone formation. "
https://pubmed.ncbi.nlm.nih.gov/2982316/

Horseradish peroxide (together with thyroid hormone) oxidizes NADH Back into NAD.
https://pubmed.ncbi.nlm.nih.gov/192281/

So this means that not all cruciferous plants have a goitrogenic effect?

It’s a root
So that is relevant

what about radishes?)

BioEclectic

@Amazoniac

Am referring to this horseradish peroxidase post from another thread:
https://bioenergetic.forum/topic/1371/biohacking-by-nathan-hatch-f-portion-control/78?_=1720359937334

@Amazoniac said in BIOHACKING by Nathan Hatch, "F*** Portion Control":

The horseradish peroxidase used in the only backup material linked was for assisting in the detection and measurement—Polymyxin B (derived from gram-positive bacteria) is the actual endotoxin binder and inactivator of these experiments. The researchers even wrote that "horseradish peroxidase alone [..] did not bind to lipid A" and made explicit its function as a "reporter enzyme." He's misattributing the effects of Polymyxin B to horseradish (peroxidase).

I insist: be watchful with the interpretations of this author.

On the practical aspect, lots of promises and not enough demonstration. Is there any health problem that he hasn't found the cure yet?

Peatful

@alex155 said in Horseradish:

@Peatful said in Horseradish:

@alex155 said in Horseradish:

@Mauritio said in Horseradish:

If I'm reading that right horseradish should have a pro-thyroid effect. Horseradish peroxide when reacting with water forms molecules that help with thyroid hormone synthesis and conversion.

"As previously shown for the lactoperoxidase FeIV Ro compound, the CcP FeIV Ro compound was involved in the coupling and not in the iodination reactions. In contrast, the HRP FeIV o pi + compound catalyzed both iodination and hormone formation. "
https://pubmed.ncbi.nlm.nih.gov/2982316/

Horseradish peroxide (together with thyroid hormone) oxidizes NADH Back into NAD.
https://pubmed.ncbi.nlm.nih.gov/192281/

So this means that not all cruciferous plants have a goitrogenic effect?

It’s a root
So that is relevant

what about radishes?)

Yes, radishes are a root too of course

I personally don’t recommend eating those but horseradish has such a medicinal or therapeutic benefit. I believe the benefits outweigh any risk.

This is my opinion

per Blake
The true method of knowledge is experiment

Lejeboca

@Mauritio Thanks for posting and renewing our attention to horseradish!

Eating is good (clears out sinuses, anti-bacterial, etc.)

The 1st paper you've referenced, however, considers the extract for "LPS-induced inflammation in macrophages" to concentrate the acting agents, I suppose "allyl-isothiocyanate (AITC) eﬀects, its main ITC resulting from the hydrolysis of the corresponding glucosinolate sinigrin by myrosinase enzyme".

So, indeed very small dose is needed (the highest dilution of the extract was 1/5th) to achieve the effects. The powder extract itself was prepared in a rather standard lab way. I am surprised though how weak it is:
10g:100ml | material (freeze-dried root) to alcohol ratio.

All in all, I think making a simple horseradish alcohol tincture and using it in 1-3 drop doses will be close to the study in terms of effects on LPS-induced inflammation.

Specifically: Take 20g of horseradish (middle of the) root, wash, finely chop, put into a glass jar, add 40ml of the highest proof alcohol you can find but no less than 100 proof. Cover with lid tightly and let sit in a dark place for 2-3 weeks or more. Shake from time to time. Strain (decant) and -- voilà -- the strained liquid is your horseradish tincture.

It looks like the study pre-treated cells with the dilution for 1h and then exposed to LPS for 24 hours before measurements. Based on this, as preventative, I'd take 1-3 drops of the tincture in a bit of water 1x per day.

Lejeboca

Cross-linking here my post about horseradish inhibiting tumor-cell proliferation which I posed in the Cancer tread here:

https://bioenergetic.forum/post/24447

Again small doses of extract components seemed to be used.

Mauritio

@Lejeboca interesting. Wouldn't that more or less come down to a similar amount as if you use it sparingly as a food/spice?

Im noticing a potent anti-fungal effect from horseradish as well.

Mauritio

That's an interesting theory.

"A juxtaposition of regional cuisines and recent prospective studies of fish consumption in China and Japan points to fresh horseradish and/or radish (HRR) as possible contributors to delaying age-related dementia. The hypothesis is that the inverse association found sometimes between fish intake and cognitive decline is partially due to exposure of the oral cavity to active peroxidases from HRR served in conjunction with fish. This hypothesis can be tested by specifically looking at whether HRR is consumed with fish and whether such HRR is prepared in a way that preserves activity of HRR peroxidases. It is possible that by putting active HRR peroxidases in their mouths, elderly people supplement their age-diminished salivary antioxidant capacity and break down additional hydrogen peroxide (H2O2) in the oral cavity before it can migrate into the brain, thus decreasing the incidence of brain cell death induction by chronically-elevated H2O2. Intentional exposure of the oral cavity to active HRR peroxidases could be a prophylactic for delaying dementia. Because vegetable peroxidases are inactivated by gastric juices, it will be difficult to obtain benefit from HRR peroxidases' antioxidant effect via ingestion in encapsulated dietary supplements."

https://pubmed.ncbi.nlm.nih.gov/28915962/

Lejeboca

@Mauritio
As a rule of thumb, you need more quantity of a plant as food than as an extract to achieve the same effects.
What horseradish you eat as food is on the order of grams for a single serving, I imagine, while 1-3 drops contain less than a milligram of the plant matter.

With extracts, it is easier to gauge the dosage too.

I, personally, like alcohol extracts because they are stored for a long time and are always on hand. A 30ml bottle would last for 8 months to a year in the dosage as above.

jamsey

For sinus infections, I’ve never found anything even close to as effective as colloidal silver in a nasal rinse(I use ionic but particle probably works). Don’t want to derail things, so here’s a horseradish study.

https://pubmed.ncbi.nlm.nih.gov/30895454/

“ Oral administration of AITC at a dose of 20 mg/kg bw restored the levels of sex hormones and its receptors, aromatase, cell proliferative markers and AgNORs staining near to normal levels. Molecular docking studies also supported these findings. The results suggest that anti-estrogenic and anti-proliferative effect of AITC prevent the development of DMBA and MNU-induced mammary carcinogenesis in rat.”

Mauritio

@jamsey great study thanks for sharing!

Kvothe

But then there is the fact that it is literally the most unpleasant food ever concoted by man.