@LetTheRedeemed , Ray recommends gelatin to be about 1/3 of the total amount of protein since gelatin is missing essential amino acids.
For me, the ratio has to be even lower, of about 1/4, not produce negative effects such as brittle nails and hair.

@DavidPS , the main takeaway from the paper in the OP is that sometimes you absolutely want to suppress NO (and iNOS) and sometimes you might not want to do so. The former is broadly true for autoimmune conditions and the latter is for true infections, esp. those with which the body has to deal with whatever means it can.

Thanks for the article Inducible Nitric Oxide Synthase: Regulation, Structure, and Inhibition (2020) !
It will take me some time to digest its 45 pages . The abstract, however, already points to the dichotomy, "both the dual modalities of iNOS and NO in disease states (i.e., protective vs. harmful effects)" indeed.

@happyhanneke said in I need x-rays:

I've been consuming gelatin and a carrot salad for a long time now. I'm also taking aspirin and cynomel.

To start with, I'd stop these substances, unless they are vitally needed for you.
Firstly, to start with a clean slate, and maybe add those gradually as needed.
Secondly, depending on the ratios of gelatin to other proteins, the amount of carrot salad, aspirin and cynomel doses, these substances may not be benign (!).

@happyhanneke , I agree with @LetTheRedeemed that this could be dealt with without x-rays.
I'd try to rule out hypotheses one by one: Do something for arthritis for a week, if helps continue (adjust), If not move on to another hypothesis for another week. This algorithm, of course, assumes that you research precise remedies for specific hypotheses and not throw a kitchen sink on each (because with a kitchen-sink approach, it will be hard to differentiate what works.)

@DavidPS , thanks!
The links to the book that I found via the rpf search
( http://krishikosh.egranth.ac.in/bitstream/1/2027529/1/HS1519.pdf )
didn't work for me [Site not accessible] .
But I managed to get a copy via scihub. Use 0046410236 in the search field. [This is book's ISBN.]
The annas-archive site worked for the books too,

Also, for laughs, check out Let's Eat Right to Keep Fit
Reviewed by: Robert E Shank >

@Mauritio said in Strong Sistas finally posted an interview with Dr. Ray Peat - The Basics Of Pro-Metabolic:

So by using that logic, Vitamin D and K should also be anti-heavy metal.

Re: Vitamin D -- No.
It facilitates the absorption of heavy metals from the gut.
Confirmed myself by idealab's multiple hair and nail analyses.
I have several posts on this as well as some reference research on the formally known as RPF.

@yerrag said in Strong Sistas finally posted an interview with Dr. Ray Peat - The Basics Of Pro-Metabolic:

I've been wondering why in one of his last newsletters Ray would say that when the body is in the process of drawing calcium from the bones, in an osteoclastic process, it cannot be at the same time in a full optimal mitochondrial respiratory mode where among other things CO2 is being produced.

From this paper Calcium paradox disease: Calcium deficiency prompting secondary hyperparathyroidism and cellular calcium overload
in Page 7:

Mitochondria are conspicuously affected by
Ca overload [151]. These organelles have the capacity
to store significant amounts of Ca in the form of biologi-
cally inactive amorphous calcium phosphate, but when
their buffer capacity is overtaxed mitochondrian physi-
ology is disrupted with severe reduction of oxidative
phosphorylation, resulting in an ATP deficit that in turn
diminishes the effectiveness of the Ca pump with fur-
ther cellular Ca overload. Elevation of [Ca] stimulates
Ca-activated neutral protease [7,8] and phospholipases
[6], affecting the pathophysiological process of muscu-
lar dystrophy [152] and other diseases characterized by
cellular Ca dishomeostasis [146]

@diomedes , I assume your morning coffee is before ~10am. This is the time until cortisol, the "waking hormone" is still high, so it may counteract the coffee "sleepiness" effect, which is possibly due to some theobromine in coffee.

There is much less theobromine in tea or mate. Try them if you'd like to stay awake in the afternoon

@LetTheRedeemed said in Reversal of hair loss in Mice with “sugar gel”:

EDIT: "it increases NO [nitric oxide is bad for metabolism] so according to danny roddy it is le bad." - sneedful
Any references indicating this to be true or false would be appreciated.

This review warns against high doses of D-ribose:
https://www.spandidos-publications.com/10.3892/etm.2021.9927

"In addition, high D-ribose concentrations may also cause chronic diabetes complications by other means. D-ribose may glycosylate insulin to form ribosyl insulin, activate Caspase-9 and -3/-7, trigger transcription factor NF-κB and produce intracellular reactive oxygen species (ROS) that may cause cytotoxic damage to the surrounding tissues and cells, eventually leading to the manifestation of chronic complications (63)."

So, I suppose, D-ribose may increase inducible NO (iNOS) by way of triggering NF-kappaB, which is a primary inflammatory response (see, e.g., pubmed here)

Personally, i will avoid supplementing with D-ribose due to a possible NF-kappaB trigger, even not being a diabetic.

Ethanol extract of propolis (EEP) inhibits nitric oxide synthase gene expression and enzyme activity

I long hoped there would be anything else but MBlue + Red Light to inhibit NO. And voilà: it's the caffeic acid phenethyl ester (CAPE), which is "identified as a major peak in the chromatogram of EEP".

From the abstract:
"EEP inhibited the iNOS promoter activity induced by LPS plus IFN-g through the NF-kB sites of the iNOS promoter. In addition, EEP directly interfered with the catalytic activity of murine recombinant iNOS enzyme. These results suggest that EEP may exert its anti-inflammatory effect by inhibiting the iNOS gene expression via action on the NF-kB sites in the iNOS promoter AND by directly inhibiting the catalytic activity of iNOS."
[emphasis mine.]

Preparation of extract from the paper:

Coarsely powdered propolis (100 g) was extracted with 10 volumes of 95% ethanol for 2 weeks. The ethanol extract was filtered, evaporated, and lyophilized to give the dry ethanol extract (33.3 g).

In Anti-inflammatory Effect of Propolis through Inhibition of Nitric Oxide Production on Carrageenin-Induced Mouse Paw Edema, even smaller amounts of extract were successfully used:

Because lyophilized ethanol extract of propolis used in the present study is equivalent to 18.5 mg propolis per 100 ml, 16) 1: 1000 and 1: 100 dilutions of propolis correspond to 1.85 and 18.5 mg/kg, respectively. Therefore, the propolis used in the present study was 5.4—54 times more potent than that reported by Borrelli et al. ... though there was a difference between mouse and rat.