Random, interesting studies

cs3000

@Mauritio was looking to get one a while back maybe didnt find a decent supp, in pigs it works at 2g per kg of diet at least locally in the intestines, probably less
maybe its better mixed into food like the pig studies because of the higher ph making it more stable?
getting it up intracellularly should react with h2o2 creating more oxygen to drive mito respiration if theres an excess, but

this one using catalase injected i.v raised lung catalase, they think it was indirect effect 10.1152/jappl.1992.72.3.858
(they increased it 70x in blood lol, relatively milder increase in lungs so i guess most of it gets used up before hitting cells, unless its intestinal cells which get it direct. with indirect increases elsewhere
normal catalase didnt work in the brain https://pmc.ncbi.nlm.nih.gov/articles/PMC4577289/#F004

theres a unique way to do it with lower dose by conjugating catalase with polyethylene glycol and putting it in the nose .
but idk if it does that spontaneously mixed together, looks like a lab process,
increased catalase activity >2x in lung fluid
https://www.nature.com/articles/s41598-020-60443-2#Sec6

Interestingly though feeding chickens catalase increased catalase in liver well along with the intestine, and some in blood
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.802051/full

Something in one of the pig studies fed 660mg or 280 U per kg diet, their calcium in blood went up a lot, https://www.mdpi.com/2076-2615/12/7/828
and catalase should help prevent calcification induced by h2o2

^ calcium in blood vessel cells
https://www.tandfonline.com/doi/full/10.3109/10799893.2012.672994#d1e959 through excess ROS signalling and TRPV https://pmc.ncbi.nlm.nih.gov/articles/PMC7599480/#sec4-antioxidants-09-00963

Seems its helping to prevent calcium go into cells which should be good for calcification issues / excitotoxity

https://journals.asm.org/doi/10.1128/spectrum.00654-21
nice effect on ileum villus height

, ordered some
the units are confusing though, pig / chicken studies theyre using units in the 100s for high mg or gram amounts

and this is insightful, https://www.jbc.org/article/S0021-9258(20)35618-0/fulltext even enzymatically inactive catalase can protect against oxidative stress well (hypochlorus acid which is one the most damaging molecules , reacts with it. produced by neutrophils)

cs3000

@Mauritio interesting one, more ROS in adipose stem cells from aging donors. ability to differentiate crashed. and adding catalase restored their ability to differentiate
https://www.sciencedirect.com/science/article/pii/S1873506122000575#s0100

Mauritio

This study shows that catalase and rapaymcin have an overlap in function.
Very interesting so low catalase might cause mTOR activation, reversed by rapa, an antioxidant or presumably catalase supplementation .
https://pubmed.ncbi.nlm.nih.gov/36474295/

@cs3000 said in Random, interesting studies:

Interestingly though feeding chickens catalase increased catalase in liver well along with the intestine, and some in blood

That's remarkable! I'm not sure what the HED is but if I'm right it's pretty low I think a few thousand units and most supplements have several 10k per pill.
Also: the trippling of catalase in organs might have drastic effects on health and longevity ,since those values are in line with that selegiline or ergothioneine achieve.

Check out this post: ergothioneine increased catalase by about 2.5 fold.
https://bioenergetic.forum/post/17948
So it's actually less effective at raising CAT than catalase supplementation in chickens , yet it still increases life span by 20% .

cs3000

@mauritio 1 more piece this backs the calcification part i was talking about, catalase gives a potent anti-calcification effect in vivo

https://scielo.isciii.es/scielo.php?pid=S1889-836X2017000100013&script=sci_arttext&tlng=en

Wild type vs TG with catalase overexpressed (CRF = renal failure)

** profound protection from calcification, 562ug/mg calcium in kidney without the extra catalase and with the catalase just 20ug

@mauritio so rapamycin acts down a few steps & we can get more direct through catalase , cool (but not as broadly increasing as rapamycin across cells taken directly). 1 thing i dont like about rapamycin is it induces anxiety or depression in the models after a while (too low neurogenesis maybe?), but the catalase gets to the core by the looks of it then

cs3000

@Mauritio

That's remarkable! I'm not sure what the HED is but if I'm right it's pretty low I think a few thousand units and most supplements have several 10k per pill.

dose is confusing why are they showing extreme amounts of units vs the studies at 500mg - 1g catalase as 100s of U?
,

maybe 2kg of food for the pig one i read they eat same calories as humans,
before with rat ones its worked out a few times as a human eating 1kg of food the same tho

ergothionine raised it better in the blood but maybe with catalase longer than 30d it increases more, the mda lowering is similar functionally. tho ergothionine might give a broader effect & in the brain with catalase giving indrect effect in some places

this pig one showed it raised catalase measured in the small intestine mucosa like +40% by 35 days, but the functional effect it halved the mda like the ergothionene showed too https://www.mdpi.com/2076-2607/8/3/391

cs3000

ah yeah the broiler one says 5000U per g catalase https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2022.802051/full

gonna look with a fresher head but seems extremely low amounts used?

this one puts 660mg per kg diet with 280U/g catalase https://www.mdpi.com/2076-2615/12/7/828

So target by the feeding studies is say 150u - 500u , but absolute weight probably counts some too without enzyme activity. which would be ~50mg by the broiler one going for a minimum by that. but 50mg can be very variable in enzyme activity depending on its extraction....

~600mg of food grade catalase looks safe enough in 90 day window even at very high activity units, but 6g pushing it https://pmc.ncbi.nlm.nih.gov/articles/PMC9943928/

But the studies all used very low units < 1000 daily heq, so might be worse effects going to those extremes

Gonna try 50mg split into meals , unless its a low unit one.
I should be a prime candidate for testing this