Posts made by haidut

@thyroidchor27

The 50mg-100mg 2x-3x daily is for general health, prevention, and when niacinamide serves solely as a precursor to NAD+. For already established diseases, such as heart failure, PD (or cancer, as in my ongoing study) higher doses may be needed. In higher doses niacinamide may inhibit direct NAD+ synthesis, but it also inhibits NAD+ depletion by enzymes such as PARP-1 and CD38, both of which consume a lot of NAD+ and are overexpressed in cancer and many other chronic diseases. So, the net effect of higher doses of niacinamide may still be higher NAD+ despite not acting as an effective precursor at high doses.

@CO3

Lol, nice name.
Btw, it already exists. It is called TocoVit.

The mainstream view on heart failure is that in most cases (when it is not congenital) it is a secondary morbidity caused by conditions such as diabetes, CVD, obesity, smoking, alcohol intake, etc. However, a direct causative agent and process has not been proposed so far. The study below demonstrates that a toxic byproduct of PUFA peroxidation known as 4-hydrodynonenal (4-HNE) is directly responsible for the cascade that results in heart failure. Reducing the levels of 4-HNE was able to actually reverse the already established heart failure. The drug used in the study is a patented chemical that increases the metabolism of 4-HNE into a less harmful substance, but I think it is obvious that a better approach would be to avoid synthesizing 4-HNE altogether. That can be achieved by restricting dietary PUFA while also supplementing with vitamin E, which largely prevents the peroxidation of PUFA into not only 4-HNE but also other toxic byproducts. Now, since 4-HNE is an aldehyde and as such is deactivated by the family of enzymes known as aldehyde dehydrogenases (ALDH), the speeding up of the activity of those enzymes can also be achieved by niacinamide or oxidizing agents, since the main co-factor of ALDH is NAD+. In addition, androgens have been shown to increase the expression of ALDH while estrogens lower it, which may explain why men with heart failure invariably present with symptoms of hypogonadism often combined with high estrogen. As such, supplementation with androgens and/or usage of anti-estrogenic substances such as aspirin, progesterone, vitamin E, vitamin K, etc may also be a viable prevention/remedy and in fact most of those substances have already shown good results in animal models of heart failure.

https://doi.org/10.1093/eurheartj/ehad662
https://newatlas.com/medical/drug-reverse-mitochondria-malfunction-mitigate-heart-failure/

"...“When a car engine isn’t running properly, energy conversion is impaired, efficiency drops, and pollution increases,” said Julio Ferreira, co-corresponding author of the study. The ‘pollutant’ Ferreira is referring to is the toxic aldehyde 4-hydroxynonenal (4-HNE), a byproduct of mitochondrial dysfunction in heart failure. “Every cell has hundreds or sometimes thousands of mitochondria, which produce enough aldehyde to poison the entire cell when they aren’t running properly,” Ferreira said. “We discovered in this latest study that too much of 4-hydroxynonenal switches off a vital event for the cell: processing of microRNAs.”"

"...microRNAs (miRNAs) are small, non-coding RNAs that play an important role in gene regulation. Disruption of miRNA formation is associated with several diseases, including cancer, neurodegenerative and cardiovascular disorders. Using mass spectrometry, the researchers observed that 4-HNE irreversibly binds to and inactivates Dicer, an enzyme encoded by the DICER1 gene essential to miRNA formation. It’s a mechanism that hadn’t been seen before. “In this study, we identified the chemical alterations that inactivate Dicer in rodents and humans owing to the accumulation of aldehyde caused by heart failure,” Ferreira said. “This was a hitherto unknown mechanism. The point is that Dicer is a limiting enzyme for formation and maturation of the microRNAs responsible for overall control of cellular biology.” Using a drug called AD-9308 on samples of human heart tissue, the researchers were able to restore Dicer activity and reverse the effect of heart failure, improving cardiac function in rodent models. In a previous study, AD-9308 was shown to activate mitochondrial aldehyde dehydrogenase 2 (ALDH2), the major enzyme that detoxifies 4-HNE, to effectively treat cardiomyopathy in mice."

@Dakota

Bingo. And there are lot more posted there, including on B1 and B3 specifically for cancer. So, if the latest experiment gets replicated in a bigger study (upcoming) then I think there is a very solid background of evidence that can be cited as a justification/explanation of why simple vitamins can be so effective. As Ray said, at higher doses every isolated substance can act more or less like a hormone, and the combination of these vitamins happens to address the main metabolic blocks in cancer (as well most other chronic conditions actually). It would be a hard fight, but medicine needs to change its attitude on "vitamins" as those are only vitamins at lower doses. At higher doses , and used together, they become potent metabolic drugs.

@chrome

Yeah, I still post there and think of it as an act of good will. Also, there is a LOT of info accumulated there and I don't want it do suddenly disappear. Disagreement over diet/ideas aside, I think it would be better for everybody if the info there stays publicly available. However, if the current anti-retinol movement grows stronger it may eventually become incompatible with the bioenergetic theory. In such a scenario, I think the forum owner himself will probably change the forum name and the forum direction officially, at which point there will be no point to keep posting.

@Mauritio

The addition of biotin was definitely a major step forward. First, I tried thiamine and niacinamide only. There was an effect but it was just delaying growth and ultimately the result was still lethal, despite doubling or tripling the lifespan of the treated animals. Adding biotin led to basically stopping growth, then there was even regression of tumor size and then the growth stayed flat, but there was no cure. Adding aspirin produced the rapid and complete disappearance seen on the graph. Aspirin by itself at the same dose, and in combination with quinine, had the same effect as the B1+B3 (without biotin).

@RePeat

Yep, one of the reasons why I included both. There are studies for each individually regarding glucose oxidation and CO2 levels, and there is also a study showing synergistic effects when combined.

@ElijahKrings

I already did a prior experiment with the same tumor line but the B1 used was prosultiamine instead of thiamine Hcl. The results were identical with the thiamin Hcl experiment. So, I decided to use thiamine Hcl going forward precisely because it is so cheap and widely available and it is legally almost impossible to ban. Prosultiamine can easily ve declared a drug and pulled off the market. It has already happened in several Asian countries with other B1 analogs such as sulbutiamine.

@ah

Yes, it will be tried again with 3 groups of 5 mice each - one control, one "standard of care" treatment, and one with the vitamins/aspirin. The initial studies are all small to keep costs low and to discover what MAY work. There is no need to do 50 mice, in animal studies 5 mice per group is enough and if the second study also shows complete cure while the control and standard group all die, then it is hard to argue it is an anomaly, especially if it is a repeated occurrence/result. Also, I will try a few other cancer types and different species (hamster, rats, etc) and if it works there too, then it is pretty clear the mechanism is very broad/systemic and not tumor-dependent and not species-dependent, in which case it becomes almost irrefutable that whatever mechanism this works through is very systemic and also that cancer is NOT a genetic disease.

@fd

IP5 has impressive reports, including complete reversal or metastatic/terminal melanoma in a human patient.
https://pubmed.ncbi.nlm.nih.gov/30615010/

But if the protocol works, there is no need to add another ingredient. Occam's rule and all that. Though it is nice to have a list of alternatives to try if the effect is not strong enough in some people.

@LaneswapSlides

The 6g+ Ray mentioned was when aspirin it used by itself. However, the combination of the B vitamins, in those doses, has a number of effects that are strongly anti-cancer and would allow for lower aspirin dose to be used. Vitamin B1 is a PDH activator, and also carbonic anhydrase inhibitor, both of which have been shown to be therapeutic in cancer. Niacinamide raises NAD+/NADH ratio, is anti-inflammatory, and inhibits excessive fatty acid oxidation, both of which have been shown to help with cancer. It is also a PARP-1 and CD38 inhibitor and those pathways are already used to treat cancer. Finally, biotin has been shown to raise CO2 levels, bypass any blockage in PDH and improve mitochondrial function, which is why a high-dose biotin (300mg) is currently in human trials for multiple sclerosis, huntington disease, and I diabetes all of which share many commonalities with cancer.

@Dakota

I answered the dosage question in a previous comment/response. The biotin dose may look large, but it is actually lower than the doses currently tried in human studies for multiple sclerosis or Huntington disease (300mg daily). In fact, I tried a higher dose biotin with the same cancer line and the results were not better, but actually slightly worse. So, it is all based on prior studies published by others, current clinical trials, and also my own experiments with lower/higher doses until I find out what works best for each vitamin.

@Vineland

There are studies on each of these vitamins for cancer or other serious diseases, so I based the dosing on that, but also tried a few experiments with lower and higher doses and this is the dose for the vitamins that worked best. The aspirin dose is also based on an animal study with cancer, I think it was liver cancer, and the animals were fully cured.

Yay, my first post here!
Wanted it to be about the recent study I did. While the results will have to be replicated with a follow-up experiment, with more animals, and additional groups for comparison, this results has never been seen before with this tumor type, which is 100% lethal and has no known case of spontaneous regression (i.e. disappearing on its own, without treatment). Basically, 2 out of 3 animals were fully cured in this experiment/study.
https://twitter.com/haidut/status/1751716166387597730

Human-equivalent doses for the substances were ~15mg/kg vitamin B1 (thiamine Hcl), 30mg/kg vitamin B3 (niacinamide), 1.5mg/kg vitamin B7 (biotin), and 15mg/kg aspirin. Administration was once daily, orally.