I don't take C routinely and I never really felt any benefit even with "heroic" doses that cause diarrhea. I do take it when I take aspirin, a few grams.

@Mauritio said in Methionine/Cysteine restriction increases longetivity AND energy expenditure:

This study shows that sarcosine lowers brain serotonin, while increasing GABA.

I took 500mg today. It was quite nice. My mood increased and my muscles looked fuller , also my skin and hair looked better . As if I had taken a big dose of collagen.
So far so good. It wasn't too exciting but it also wasn't sedating so actually a nice balance.

"Pretreatment of sarcosine (600 mg/kg, i.p.) non-significantly improved learning and memory deficits induced by non-competitive NMDA receptor antagonist MK-801, significantly increased the GABA and decreased the 5-HT levels (p<0.05)."
https://pubmed.ncbi.nlm.nih.gov/25710578/

thank you -- great find.

@Mauritio Sarcosine seems to be a mixed bag.

https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1878-0261.12439

DNA hypermethylation is one of the most common epigenetic modifications in prostate cancer (PCa). Several studies have delineated sarcosine as a PCa oncometabolite that increases the migration of malignant prostate cells while decreasing their doubling time. Here, we show that incubation of prostate cells with sarcosine elicited the upregulation of sarcosine N-demethylation enzymes, sarcosine dehydrogenase and pipecolic acid oxidase. This process was accompanied by a considerable increase in the production of the major methyl-donor S-adenosylmethionine (SAMe), together with an elevation of cellular methylation potential. Global DNA methylation analyses revealed increases in methylated CpG islands in distinct prostate cell lines incubated with sarcosine, but not in cells of nonprostate origin. This phenomenon was further associated with marked upregulation of DNA methyltransferases (Dnmts). Epigenetic changes were recapitulated through blunting of Dnmts using the hypomethylating agent 5-azacytidine, which was able to inhibit sarcosine-induced migration of prostate cells. Moreover, spatial mapping revealed concomitant increases in sarcosine, SAMe and Dnmt1 in histologically confirmed malignant prostate tissue, but not in adjacent or nonmalignant tissue, which is in line with the obtained in vitro data. In summary, we show here for the first time that sarcosine acts as an epigenetic modifier of prostate cells and that this may contribute to its oncometabolic role.

@Sippy I don't know. But I would probably try it. Dr. Stephens may actually reinforce this, who knows, because he has used it apparently with people who had all sorts of brain issues. He really points to all problems as originating in the brain, resulting in hyperglycolysis, resulting in throttling of glucose, resulting in...all sorts of mental illness and issues. So yeah. Yeah. And I can't see how it would cause harm.

methionine restriction is good. Also consuming methioninease is better. The enzyme which is present in many animals and molds, is now patented and very expensive, but it can result in a tumor completely disappearing as it gets rid of almost all methionine.

@CrumblingCookie that is a huge dose of folate.

I’m at 70g four times daily. Taking with potassium rich beverage. Gained a few pounds. Maybe just water weight. My temperatures are not any different. I am working to reduce headaches, and I think they are reduced, but they are not gone. Still hopeful.

I’ve not been that impressed with rapamycin personally, as it gives me a headache and interferes with sleep.

But low dose naltrexone and deprenyl are quite amazing and are great longevity drugs. LDN in particular has made a huge different for so many people as it can seal leaky gut — which is the source of virtually all chronic disease as we age.

@GRay carbonic anhydrase inhibitors and beta blocker drops are safest. Prostaglandin drops not so much. The “drops” I make work through carbonic anhydrase inhibition. My loved one had a laser iredectomy but it isn’t enough. Not sure it is actually useful.

tea is very high in fluoride. The more expensive teas are harvested from young shoots, which are much lower in fluoride due to less time growing to accumulate it. But that is the issue, the cheaper assam teas can have 2mg per cup.