Posts made by Mauritio

@DavidPS said in A combination of vitamin B1/B3/B7 and aspirin, has curative effects on human mantle-cell lymphoma:

@Mauritio - Yes, it appears that he is not interested in patenting.

He selected the least expensive form of B1 to make his invention accessible to as many people as possible.

I am a registered patent attorney. I think he could obtain a patent based on the limited facts that I have. He solved a long-standing problem; that is a strong indication of patentability.

Ok in that case ,you know more about this than me.
Would be an interesting thing to ask him if he has any intentions on patenting it.

@Mauritio said in Methionine/Cysteine restriction increases longetivity AND energy expenditure:

Concentric exercise, in this case, treadmill uphill walk variations, increase FGF21 by 66% in 2 weeks in humans
https://pubmed.ncbi.nlm.nih.gov/22701542/

Interestingly upstairs walking, a form a concentric exercise, has by far the highest calorie expenditure amongst all the sports tested.

https://x.com/T3MaxxiAlt/status/1759793246769987880

BTW if anyone finds any other ways to boost FGF22 let me know. I feel like boosting FGF22 is a better way key to get the benefits of MR without having to eat in a too restricted way.
I think methionine restriction boosts FGF22 something like 800% and I have yet to find anything else that comes even close to that .

It might be that he is waiting for a publication, but Im not sure if he really wants to patent this treatment. Did he say that anywhere ?
Plus, I'm not sure if you can patent a combination of basic vitamins. Normally you would have to change something structurally on the molecules ...

All 3 mice are now fully cured and cancer-free.

I dont know why Georgi isn't making a bigger deal out of this. Because this is a big deal.

https://x.com/haidut/status/1758122576411279659

https://x.com/haidut/status/1777337478636007790

@wrl said in Ergothioneine - amino acid for life extension.:

Ergothioneine: A Stress Vitamin with Antiaging, Vascular, and Neuroprotective Roles?

https://www.liebertpub.com/doi/full/10.1089/ars.2021.0043

Good one.

It also seems to have cosmetic benefits .

"ET accumulates in skin cells and not only prevents oxidative damage but also facilitates DNA repair in UV-irradiated cells (80). For these reasons, ET has been included as an ingredient in several skin care products and cosmetics."

I took my first tablet yesterday.

It increases muscle fullness and I am feeling more androgenic. I think it is good for liver health. Had some bile dumps and felt a sense of relief after.

I am currently adjusting my thyroid dosage and everbody who did that before knows that can be a pain in the ass and you might have to go through a few days of suboptimal thyroid function. Ergothioneine definitely helped with that and relieved the low mood and lethargy associated with hypothyroidism. It also made me more active during the day, but sleep was very refreshing.
Verbal fluency seems increased.
The only downside is hair loss is a bit increased. But not strongly.

I can only encourage people to try it. Given its long half life it might be taken only 1 or twice a week since the body accumulates it similarly to the fat soluble vitamins.

@Peatly said in Mild Vitamin A deficiency decreases DHEA and Pregnenolone by 40%:

@Mauritio Thank you.

I just found this quote from the mega master newsletter

"Recently, the polyunsaturated oils have been identified as the main thing in cells that radiation interacts with, to cause cellular damage. Vitamin E, taken internally or even applied to the skin, has been found to reduce the damage produced by exposure to ultraviolet radiation, which is logical, since it interrupts the chain reactions of toxic free-radicals produced when unsaturated oils are oxidized by radiation or other injury. Aspirin has been found to have a similar effect in reducing the harmful effects which develop in the skin after sunlight overexposure. Coconut oil has been used for generations in “ suntan lotions, ” and whether it is absorbed through the skin or eaten as a food, it clearly has a protective antioxidant function. Carotene seems to work with vitamin E in the skin to reduce injury by ultraviolet radiation. Caffeine also has shown a protective action against radiation, but its mechanism of action isnʼt clearly understood." Townsend Letter for Doctors & Patients, June 1996, Page 83 – 85

Dr. Peat also said he stopped burning in the sun after supplementing vitamin D, getting lots of milk and limiting PUFA.

Good one.

@Peatly said in Mild Vitamin A deficiency decreases DHEA and Pregnenolone by 40%:

UV Radiation Induces Vitamin A Deficiency in Skin

Peat has a very old newsletter where he says that UV rays cause a film of free radicals on hair (and skin) that can persist for a few hours ,even after leaving the sun. Just a few seconds of red light will neutralize the free radicals.

Thanks for mentioning. I forgot about taking it. It made me kind of wired but tired after a while ,but I did enjoy it. And might try it again.

ERGO can protect against social defeat.

"...ergothioneine prior to and during the SDS (social defeat stress) paradigm had a preventative effect on SDS-induced depressive behaviors, such as social avoidance and depression-like sleep abnormalities, particularly those of rapid eye movement sleep."

Not only that, I already mentioned that some gut bacteria can synthesize ergothioneine. One of them is L. Reuteri. It can increase testosterone dramatically and is anti-inflammatory. Well, another benefit can be added to the list now! Hans made an interesting video on it.

https://www.youtube.com/watch?v=UuOxSrFonqs

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256047/

T3 increases FGF21 as well.
https://pubmed.ncbi.nlm.nih.gov/20236931/

I always suspected there was more to semaglutide than it lowering appetite.

It increases FGF21 sensitivity. So in a way it is mimicking methionine restriction.

In many diseases FGF21 is high (I assume as an adaptive response), but similarly as in diabetes and insulin, their body is resistant to it. So there is such a thing as FGF21 resistance, which is ameliorated by semaglutide, leading to weight loss.

• https://pubmed.ncbi.nlm.nih.gov/36905134/

• https://pubmed.ncbi.nlm.nih.gov/28525362/

Protein restriction might be the best option to increase FGF21 and thus longevity, but not the easiest. Something that everybody can do at home and that Peat had recommended for decades is to eat lots of carbs.

This human study shows that Increasing carb intake is way more effective at increasing FGF21 than incresaing fat intake.
The protein intake was the same during the whole study.

The FGF21 concentration increased 8-fold in the high carb group!

"After CHO, plasma FGF21 concentration increased 8-fold compared to CON (329 ± 99 vs. 39 ± 9 pg ml-1, p < 0.05)."

So protein restriction might not even be necessary.

Another interesting fun fact is that the high carb diet increased the concentration of palmitoleic acid by almost 150%. Its interesting that when the body makes new fat that it is a pro-metabolic MUFA. Palmitoelic acid is contained in macadamia nuts a lot.

• https://pubmed.ncbi.nlm.nih.gov/28123934/

Great video, thanks for sharing.

Wow.

"...MRD (0.17% methionine + 1.20% choline) for 90 consecutive days. We found that MRD reduced body weight and fat mass; increased heat production and ambulatory locomotor activity; reduced hepatic and plasma lipid levels, hepatic fatty infiltration area, and adipocyte volume in white and brown adipose tissue; promoted fat browning, especially upregulated gene and protein expression levels of uncoupling protein 1 (UCP1); and promoted fat catabolism and inhibited fat anabolism in the liver and adipose tissue. Moreover, MRD increased antioxidant defenses and reduced inflammatory cytokine levels in the thyroid, blood, and liver. Furthermore, # MRD improved thyroid morphological structure, promoted the synthesis and secretion of thyroid hormones, and enhanced the actions of thyroid hormones on its receptor organs (liver and adipose tissue). These findings suggested that MRD promoted fat browning and attenuated hepatic lipid accumulation in HCD mice associated with the improvement of thyroid function."

• https://pubs.acs.org/doi/10.1021/acs.jafc.2c05535#

"Vitamin A and D deficiencies are associated with immune modulatory effects and intestinal barrier impairment. However, the underlying mechanisms remain unclear. C57BL/6J mice were fed either a diet lacking in vitamin A (VAd), vitamin D (VDd) or a control diet (CD) for 12 weeks. Gut barrier function, antimicrobial peptide (AMP) defense and regulatory pathways were assessed. VAd mice compared to CD mice showed a reduced villus length in the ileum (p < 0.01) and decreased crypt depth in the colon (p < 0.05). In both VAd- and VDd-fed mice, ileal α-defensin 5 (p < 0.05/p < 0.0001 for VAd/VDd) and lysozyme protein levels (p < 0.001/p < 0.0001) were decreased. Moreover, mRNA expression of lysozyme (p < 0.05/p < 0.05) and total cryptdins (p < 0.001/p < 0.01) were reduced compared to controls. Furthermore, matrix metalloproteinase-7 (Mmp7) mRNA (p < 0.0001/p < 0.001) as well as components of the Wnt signaling pathway were decreased. VAd- and VDd-fed mice, compared to control mice, exhibited increased expression of pro-inflammatory markers and β-defensins in the colon. Organoid cell culture confirmed that vitamins A and D regulate AMP expression, likely through the Jak/STAT5 signaling pathway. In conclusion, our data show that vitamin A and D regulate intestinal antimicrobial peptide defense through Wnt and STAT5 signaling pathways."

• https://pubmed.ncbi.nlm.nih.gov/36678247/

" TPH1 is highly expressed in SBNETs and many cancer types. TPH1 knockdown cells and telotristat treated cells showed similar growth rates as control cells in vitro . However, TPH1 knockdown cells formed smaller tumors in vivo and tumors were less vascularized. Although Tph1 inhibition with telotristat showed no effect on tumor cell growth in vitro , Tph1 inhibition reduced tumor formation in vivo . Serotonin inhibition in combination with other therapies is a promising new avenue for targeting metabolic vulnerabilities in NENs."

• https://pubmed.ncbi.nlm.nih.gov/37066322/

@Kasper said in Methionine/Cysteine restriction increases longetivity AND energy expenditure:

FGF21

FGF21

The benefits of MR are almost exclusviely downstream from FGF21. ("...mice lacking FGF21 fail to exhibit metabolic responses to protein restriction...")
https://pubmed.ncbi.nlm.nih.gov/35393401/
Interestingly FGF21 fails to have effects when the longevity gene klotho is silenced, so FGF21's effects seem to depend on klotho.
https://pubmed.ncbi.nlm.nih.gov/23209629/

• Ketogenic diets decrease FGF21 (and klotho) as per this study, so they are not good for replicating MR
  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4431718/

• Low protein diet increases FGF21 more, but high protein diet decreases liver fat more
  https://raypeatforum.com/community/threads/high-protein-diet-reduces-hepatic-fat-than-low-protein-diet.35461/

• Low carb, high protein diet decreased FGF21 by 54%
  https://pubmed.ncbi.nlm.nih.gov/38379856/

• Molecular hydrogen increases FGF21
  https://pubmed.ncbi.nlm.nih.gov/21293445/

• high sucrose diet increases FGF21
  https://pubmed.ncbi.nlm.nih.gov/28886439/

• Concentric exercise, in this case, treadmill uphill walk variations, increase FGF21 by 66% in 2 weeks in humans
  https://pubmed.ncbi.nlm.nih.gov/22701542/

@hypercashoidism said in The pro-metabolic effects of apple polyphenols:

@Mauritio i'm still not sure since i remember reading that 'theory of everything' reddit post a while back which theorised that procyanidin works through increasing intracellular degradation of DHT through the increase of an enzyme i forgot the name of. In that case i'm not sure if that's something i want, i'm more interested in better eyebrows and facial hair which more DHT breakdown would antagonise

Balding is a metabolic disease. And this thread offers 10 different mechanisms on how procyanidin helps with that, if that's not enough and you rather rely on some post you read some time ago that you vaguely remember, then I dont know what to tell you.

"...hippocampal neurogenesis were lower in mice fed an ERGO-free diet than in those fed the control diet. Furthermore, ERGO supplementation to achieve the control diet ERGO levels reversed these effects and restored ERGO concentrations in the plasma and hippocampus."

https://www.nature.com/articles/s41538-024-00250-5