Posts made by Mauritio

Here's a guy on reddit who claims to have cured his balding by using anti fungal spray on his head. Even included some decent pictures.

https://www.reddit.com/r/bald/s/SMe3lriWFf

@Nyck-Star-Gel Super danke werde ich mal probieren.

@cs3000 Nice ! You might want to send this to Georgi , maybe he'll do a small study on it.

@Gaston said in Reversal of hair loss in Mice with “sugar gel”:

You guys are cookin with gas now, I can't understand what the hell you're talking about at this point. Keep it up.

@alfredoolivas said in Reversal of hair loss in Mice with “sugar gel”:

I was thinking, thank god I don't have hair loss, so I don't need to dwelve deep into this research. These guys are onto another level lol, much respect.

Lol thanks guys.
Great teamwork on this thread. This could help many, many people if it works out.

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

This indicated that, consistent with Flores et al., anaerobic glycolysis is required for HFSC activation, but it does not influence HFSC to ORS lineage progression."

That's good to know.

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

Nice follow up on this! I am unsure, however, whether these effects are mediated through HDACi or whether they are separate effects on Akt activation->GSK3beta inhibition->Wnt activation. It may be a distinct mechanism?

I'm not sure.im not deep into the HDAC literature. I just recall from one of the studies I read today that they said it was am epigenetic mechanism stabilizing the pro-hair genes .

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

so, topical lactate? If the topical solution remains at pH <5 we may even use benzoic acid or benzoate at 0.5-0.9% as a preservative instead of the high 35% ethanol. Maybe combined with an equimolar amount of glycine to allow for its enzymatic conversion to hippuric acid once applied and absorbed.

Lol I've been peating too long to voluntarily apply lactic acid to my scalp, I feel safer with giving my scalp the raw material(s) ,mainly dextrose, and then let it figure it out.

Why do you think it's less than 5 ? I haven't calculated it but I guess it should be higher . Especially once adding potassium bicarbonate. Why benzoic acid?
The ethanol is not just there as a preservative but also as a solvent and absorption enhancer . The apple polyphenols are poorly water soluble IIRC but better soluble in ethanol for example. So benzoic acid would have to be able to do these things as well.

@AinmBeo said in Reversal of hair loss in Mice with “sugar gel”:

Does the "Ethanol" listed here imply pure alcohol (no water in the mix)?

Yes.

I've updated tie formula and added sodium butyrate. The amount needed is surprisingly small, and I've already taken a couple times more than needed. This is based on one in vitro study so very speculative, but I've read something about it increasing osmotic stress if you exceed 10mM, not sure if that's true/relevant.

New formula:

D-Glucose: 1%
Apple Peel Polyphenols: 1%
NMN: 1%
Sodium Butyrate: 0.075%
Ethanol: 35%
Water: 65%

@AinmBeo haidut always says NMN is basically niacinamide, which I don't think is true. Not in hair and not in all of the rest of the body . He's very pro niacinamide so maybe he's a bit biased there.
Not saying Niacinamide doesn't have benefits , hope I'm clear on that.

@Mauritio said in Reversal of hair loss in Mice with “sugar gel”:

So lactate itself might be wanted and needed for hair

Here's an interesting review. Under 3.4.3 they talk about lactate and hair loss.

Lactate increases VEGF, leading to new blood vessels and better nutrients supply.
Also, lactate can increase glycogen levels, which increases anagen phase.

https://pmc.ncbi.nlm.nih.gov/articles/PMC11879785/

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

Sorry I had missed this post. It's essentially the same study and authors we both dug out there.

No problem .

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

The "spatiotemporal" effects really open up a different angle to determine suitable combinations and timings of different topical substrates. We wouldn't want a limited phase of fully engaged "hair growth bloom" followed up by depletion of the underlying stem cells and exacerbated balding.

Totally agree. Gotta look more into LONG term studies on these substances , although they're probably scarce.

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

We also observed glycolysis-mediated histone acetylation is increased and chemical inhibition of acetyltransferase reduces expression of the signature genes associated with hair induction in active DP. "

Good one! Another reason to use butyrate and other HDACs.
The study even cited 2 other studies showing beneficial effects of HDACs on hair.

https://pubmed.ncbi.nlm.nih.gov/30814580/
https://pubmed.ncbi.nlm.nih.gov/22506014/

@CrumblingCookie said in Reversal of hair loss in Mice with “sugar gel”:

DP also rely on aerobic glycolysis. So no obvious contradictions between HF and DP so far.

But they study cited another one showing that HFSC rely on ANaerobic glycolisis, generating large amounts of lactate (not pyruvate)
So lactate itself might be wanted and needed for hair (stem cells).. something I never thought I'd say.
Or at least less lactate = less hair stem cells

But is their a cost?
Meaning more stem cells = less actively growing hair.
I think the glutamine study hinted at outer root sheath (ORS) cells reverting back to HFSC, which should reduce the hairs in anagen phases.

Then we're running into the glutamine problem again.
Short term vs. long term effects.

https://pubmed.ncbi.nlm.nih.gov/28812580/#:~:text=Furthermore%2C lactate generation appears to,(Ldha)%20prevented%20their%20activation.

Although this study suggests there is no difference in lactate between people with hair loss vs. No hair loss.
Which at least suggests high lactate there is normal and not necessarily a sign of a broken metabolism .

Squalene, a major component of sebum, was higher in people with hair loss, suggesting higher sebum production.
https://pubmed.ncbi.nlm.nih.gov/37760935/

Glutamic acid.
Not exactly glutamine, but related .

Has very impressive hair boosting effects in this study, especially the 1% and 10% solution .

https://www.nature.com/articles/s41598-021-94816-y/figures/3

@CrumblingCookie I haven't fully read it, but I feel like this study explains a lot of what we're wrestling with.

It seems that glutamine drives hair follicle growth from stem cells (HFSC) to outer root shaft cells which support the growing hair in the anagen phase. Interestingly this process is triggered by glutamine inhibiting aerobic glycolisis and boosting TCA cycle. So generally this could be achieved by substances that supress glycolisis and boost TCA cycle.

On the other hand they show that excessive glutamine or blocking mtorc2, long term depletes the HFSC pool, and I guess over long time frame that would lead to impaired regeneration and thinning hair.

This is also explains why they saw such drastic effects in the study above, but I'm not sure if that would be the same long term.

So, maybe cyclic addition of glutamine would be best. Between 1-10% , maybe 1 month on 2 months off or sth like that ?

https://pubmed.ncbi.nlm.nih.gov/32905798/

@AinmBeo I'd use NMN if possible.

Topical niacinamide does not stimulate hair growth based on the existing body of evidence
https://pubmed.ncbi.nlm.nih.gov/31955438/

Sure that's kind of the point.

@CrumblingCookie I discussed the hair metabolism in this post
https://bioenergetic.forum/post/41937

I've read the study you linked.
And I'm not sure glutamine is necessary . It gives a small energy boost, but maybe it also has it's draw backs (ammonia, inflammation?) .

It does increase total ATP content, but it also inhibits glucose utilization, indicating that they're competing for oxidation. Maybe glutamine is an emergency fuel.

Also glutamine is being converted to a ketoglutarate, which can feed the TCA cycle . But as we know this is a minor source of energy in the HF so I wouldn't focus on it too much . And it's partially taken care of by the current formula already.
But I'm gonna look into it more.

In wondering what other more benign amino acids do for the hair, like glycine ?

@dopamin3 i think we chatted briefly on x already.
That you can't find anyone like minded In a city as big as Berlin Is wild. But Berlin people don't strike as the average peaters lol

@AinmBeo No I just leave it in. This should increase absorption .
It's surprisingly practical. Even the sugar version is growing on me. Its sticky first, but after drying it works a little like a hair gel.

Which version are you using ?

Im gonna add sodium butyrate this weekend .

Sulforaphane

Increases FGF21. Ameliorates NAFLD.
https://pubmed.ncbi.nlm.nih.gov/34654875/

Lowers weight gain on a hfd, increases UCP1, lowers scd1
https://pubmed.ncbi.nlm.nih.gov/33995092/

Lowers 5-HT(3A), 5-HT(1A), 5-HT(2C) in vitro
https://pubmed.ncbi.nlm.nih.gov/18593952/

Increases PGC1a
https://pubmed.ncbi.nlm.nih.gov/35955572/

And several impressive human studies on autism. Probably helps because of anti serotonin effect.

Activates FXR ,helps liver health.
https://pubmed.ncbi.nlm.nih.gov/36448414/

@Milchkanne Wenn das die Milchkanne sagt ?!

Nice danke. Ich hatte bis jetzt nur die in der durchsichtigen Flasche, wo gibt's denn die braune ?

@AinmBeo said in Reversal of hair loss in Mice with “sugar gel”:

Or getting the hair wet and making "rows" and applying it to the scalp at the base of each row?

This without making it wet. I apply it to the scalp, massage it and spread it with my fingers and after that comb my hair to spread it and increase circulation.

@Hearthfire I have used it orally.

I think heavy metals can cause fungus overgrowth since they bind and incorporate heavy metals. And we're certainly getting heavy metals from all kinds of angles.

@CrumblingCookie thanks for sharing. Great read.