Posts made by cs3000

@Mauritio its mostly inhibiting just the variant there (not normal androgen receptors) splice variants lack parts that de-activate them so continually active https://www.nature.com/articles/nrurol.2015.13

ironically depleting androgens in prostate cancer can induce more of them (and more significant amounts in the extracted cells part but idk how much they contribute)
https://aacrjournals.org/clincancerres/article/20/6/1590/211454/Rapid-Induction-of-Androgen-Receptor-Splice

AR-FL (normal receptors) arent getting inhibited much but AR- variants gone

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1 found mild estrogenic activity in vitro in cancered cell lines which doesnt match with the in vivo anti tumor activity,
but mainly on ERRy and ERRa estrogen related receptor, 30% max transcriptional activity of estradiol on estrogen receptors, (maybe displacing estrogens for milder effect lower transcription? but potent on ERRs in these cells)
,

https://www.sciencedirect.com/science/article/abs/pii/S0300483X21001281

They found some hormone reducing effects in fish but idk how relevant to mammals

In adrenocortical cancer cells it reduced hormones production at nM concentration,
https://academic.oup.com/jcem/article/103/10/3706/5056322

• It has a mutagenic effect at higher end dose

humans show lymphocyte abnormalities from treatment at higher end dose. apparently its lethal in 100% of some strains of mice injected at low dose 7.5mg/kg. needs to be transformed in the gastrointestinal tract first & go through the liver https://pubmed.ncbi.nlm.nih.gov/3278217/

orally 60mg/kg ~300mg-400mg human dose starts to give a rise in abnormal sperm

its a mild effect there but thats only with 5 consecutive days

in humans the mutagenic effects on lymphocytes with 1g-2g for a day then 6 days of 500mg increased. (i'd guess red blood cells might be more vulnerable) doi: 10.1016/0165-7992(86)90015-1)

so to be on the safer side i wouldn't take >100mg of this regularly, 25mg has effects. and maybe good to have a few days off a week just incase.

@Mauritio said in Niclosamide reduces serotonin and glutamate:

Didn't you write something about cancer and pH? Niclosamide seems to affect that :
https://pubmed.ncbi.nlm.nih.gov/22474287/

cool thanks , yeah lowering pH to a certain level helps make the apoptosis process more effective

@Mauritio wow impressive one thanks. how are you taking it & what do you notice, if you still do? so far im thinking day on day off, after bowel movement, maybe pm use, 25mg-50mg, or to have handy for a serotonin state occasionally

thats a big effect on serotonin induced itching (could be very useful for lightening the effects of mast cell activation syndrome , as thats not just histamine mediated its through serotonin and others too via the TRPv trpa receptors). thats the best effect ive seen for this

an upside of only needing a small amount of a normal tablet assuming cant get powder, u get a lot less of all the shite its mixed with

it decreases the clearance of some compounds through inhibiting some kidney transporters, something to be aware of (& maybe day on day off is best)
https://www.sciencedirect.com/science/article/abs/pii/S0009279724000322

another reason should be good for cystic fibrosis, (on top of the anti fibrotic effect u mentioned) lowers the mucus secretion toward controls https://pmc.ncbi.nlm.nih.gov/articles/PMC10794189/#Sec2

and showing the anti fibrosis effect (reversed fibrosis / ED in penis of diabetic rats) https://academic.oup.com/jsm/article-abstract/21/12/1111/7822244?redirectedFrom=fulltext
"Both functional and molecular alterations in DMED were effectively reversed by Nic-treated diabetic rats without a glycemic alteration. Nic could be a promising candidate for the treatment of DMED due to its antifibrotic effects."

https://aacrjournals.org/cancerres/article/71/12/4172/567782/Antihelminth-Compound-Niclosamide-Downregulates
showed a good slowing effect but not massive on fast growing tumor, but for a slow growing tumor it stopped growth for a while
(i guess tolerance built using 6 days a week for 18 days? controls surged then too so maybe not tolerance & less effective at end stage)

@gg12 https://www.reddit.com/r/EyeFloaters/comments/c9lj81/floaters_after_thyroidectomy/

@Tarzan17 hi some common recommends are carrot salad , cascara ,

for me some daily bromelain + eating a meal with some organic wholegrain for the fiber worked for the most part after many months of severe constipation (would go a week without any bowel movement). 10 minute walks i think give you a chance in right conditions,
in an emergency you can use magnesium citrate or very high salt in a big glass of water and turn into a human pressure washer for a few rounds

@highseabasch used to get it, tried to move my toes first like that scene from kill bill https://www.youtube.com/watch?v=vHl24Kjp5Vs
think i usually had to relax and accept it was gonna take a bit of time as a process underway for things to flip back online

@dapose thanks for the reminder, wanted to look into this guy since i heard peat mention his stuff on immunity without reliance on the immune system. seems like one of the grand masters . some stuff is going over my head but parts that stood out so far

• buildup of clunky large molecules (polymers) when a surplus of oxygen isnt supplied to the cell (or low amount of oxidation catalysts) , only a small amount of things that can join are taken out so more stuff combines and makes cellular process inefficient with these in the way:

• hard to burn peroxides
  

• malignancy of cancered cells and their loss of oxidizing power going together, and showing higher amounts of polymers , duplicating as attempt to dilute the molecule faster than its being added / created

• importance of carboxyl groups
  

• (interestingly he says all carcinogenic compounds have a fluorescent property)

• ? providing a competitive energy carrier with similar structure that doesn’t divert sugar / fatty acids away from being fully utilized and to cell division instead, supplying a normal oxidation carrier, that has 2 types of unsaturated groups which can competitively absorb heat and redirect energy to sugar & fat oxidation instead of hyperactivating other processes without being able to be carried far enough

• ,
  

@ThinPicking
Billions of farts must end

Farts must be stopped at all costs. billions will die

(Farts in public)
Lamp post speaker:
"Citizen 7754271 - you have - 9 - farts remaining"

@Milk-Destroyer tried ocado (m&s) goats milk and it was thicker & tasted better than others

@Insomniac said in Major retailers join forces with UK dairy farmers to trial methane-reducing feed additives:

reverse dragons, the enemy

On the bright side, one day the whole world will be cow fart safe even if dairy is no longer edible..

continued from #1 https://cs3001.substack.com/p/a-bunch-of-quick-health-and-biology

1. Surgery causes a big acute drop in pyruvate dehydrogenase activity through depleting thiamine (needed to use carbs fully, converting pyruvate from carbs to acetyl-coa & co2)
   https://pmc.ncbi.nlm.nih.gov/articles/PMC4472607/

takeaway: need a thiamine boost after surgery

Measurements of PDH activity and quantity and thiamine were obtained prior to surgery, at the completion of surgery, and 6 hours post-surgery. Study subjects had a mean 41.7 % (SD: 27.7) reduction in PDH activity after surgery and a mean 32.0% (SD: 31.4) reduction 6 hours after surgery (p < 0.001). Eight patients were thiamine deficient (≤ 7 nmol/L) after surgery compared to none prior to surgery

2. Monolaurin protects well against staph infection https://pubmed.ncbi.nlm.nih.gov/20021093/ http://visordocs.sic.gov.co/documentos/Docs019/docs20/2014/2014006996OF/2014006996OF0000000003.PDF?713
   killed all 14 control mice within 1 week. 50% survived for 30 days when given monolaurin orally.
   3.2mg monolaurin given to mice weighing 15 to 20g so 160g/kg = shown effective at 800mg-1g human dose. (higher dose might not be better).

Human milk contains monolaurin around 3 mg/mL. When the monolaurin is eliminated, the anti-inflammatory and antimicrobial activity is low

3. Constipation -
   daily physical movement (walking) plays a big role in bowel movements. inactivity for 2 weeks = bowel movements went from average of 9.5 a week -> 4.5 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748072/

Our results showed a new onset of functional constipation at the end of 35-days of bed rest in 60% of healthy volunteers as diagnosed by Rome III criteria

4. More https://cs3001.substack.com/p/some-quick-health-finds-2

@dapose i take 2.5g each night too, decent help for lightening pm stress & getting to sleep. high amounts cause gut irritation so i keep it there

u need a mild acid to react with the bicarb to create co2 for the bath , i used some malic acid
3grams of bicarb + 3.5grams of malic acid per L of water should max it out but that would be a lot for a full bath, so can just try warm water & mix in a couple tablespoons of each at a time until you see your skin turn pink

@BroJonas they do study medroxyprogesterone as if its progesterone sometimes when it isnt but for that 1st study thats progesterone (also elevates in pregnancy in supplementary materials)

which seems confusing at first but:

1. might only be relevant in high doses found in pregnancy (1mg per mouse is like 200mg-300mg highly absorbed, so 1g+ orally) ,

2. its organ specific ( basically what the study's showing is in high doses progesterone probably switches glucose oxidation in some places so there's enough glucose for the fetus to use to grow well. i bet it really kicks in when the brain is developing more near the end)

@ejmcc27 generally a sign of lower atp / hypoxia (eyelid is very common place to get twitches 1 area that shows hypoxia & hypothyroidism obviously, e.g darkens or droops down, with very high usage. atp is used in reversing muscle contraction, with twitches the muscle is failing to stay in the relaxed state),
could try stopping vitamin D and limiting UV light for a few weeks to confirm if its that making it worse. thats what i found. + eating more calories if you have low intake (fat release of stored vit d otherwise). in poor health vitamin D can excessively convert to a form that inhibits mitochondria. it could be from a range of things causing the hypoxia / lower atp though

@Mauritio said in Thymus health:

@cs3000 said in Thymus health:

Looks like the main benefit of the Thymus could actually be from suppressing too much immune damage via t reg cells

That is such an interesting thought. Why wouldnt we have an organ whos purpose it is to dampen excessive inflammation, given chronic inflammation is a part of so many diseases.

@yerrag said in Thymus health:

The funny thing is that the elephant in the room was always being ignored, in the absence of mention of the T-cells, such that it's importance was relegated to that of a bit player, inconsequential to improving our body's response to the putative virus.

Seems to me that beefing up the thymus gland should be the main topic of the COVID response. The question of the day.

Looks like a big health unlock , neutrophils are big players in autoimmune problems maybe the biggest, you see them showing up causing big damage in a wide range of health issues covid cystic fibrosis ulcerative colitis heart failure etc

and theyre highly destructive with different weapons, cytokine release, then they have proteases that degrade the structure surrounding the cell (if u inhibit neutrophil proteases in arthritis they dont develop arthritis at all https://pmc.ncbi.nlm.nih.gov/articles/PMC150852/ ), and they can form wild traps where they basically gut themselves & spill out strands of dna laced with proteins to form damaging webs like some kind of arsehole spiderman in the autoimmune situation

their benefit is mainly in the first 12 hours where theyre supposed to help kickstart the cleanup & repair process then back off (reversing their path back into bone marrow). but in the current state of health its common that they stay chronically activated for days weeks months years . regulatory t cells specifically T Reg cells look key to help resolve that

@yerrag I can't help but think of metabolic health being as impactful on our immunity, and this is is but just one example of Ray's dictum that metabolic health drives all other aspects of health in us.

something that fits with that & the rest, neutrophils dont use mitochondria for energy they basically just use glycolysis (& can create their own glucose in presence of glutamine too, and use fatty acids for some functions like ROS production or possibly switch over to fatty acid use to become more intense, in my experience eating more fat takes high neutrophil damage to an extreme),
BUT unlike neutrophils T Reg cells need mitochondria complexes functioning to enable their immune resolving function

@LucH yeah, also idk about putting a high watt device on skin because of high EMF , maybe 1 foot away is ok if intensity stays high and short duration?

@DavidPS

Ty looked into more of theirs, just 3 minutes into the eye of 8mw/cm2 (which they said is still more intensity than what sunlight gives at that 670nm red wavelength) boosted color vision for a week
https://www.researchgate.net/publication/356509034

670nm for 2 minutes 40mw/cm2 daily every morning, didnt help reverse age related eye degeneration though https://www.researchgate.net/publication/340403571_A_Pilot_Study_Evaluating_the_Effects_of_670_nm_Photobiomodulation_in_Healthy_Ageing_and_Age-Related_Macular_Degeneration

but yellow + red + near IR (majority 670nm red) at 50 - 80mw/cm2, for 1 minute 30, for 3x a week for 3 weeks, gave a slight vision improvement https://pmc.ncbi.nlm.nih.gov/articles/PMC5484346/

so yeah 3x a week maybe better than daily
seems weird though because you'd be getting red light from the sun daily

30nm LED at just 3 mW/cm2 at skin level (created 1cm2 light spot on fifth ribs) improved LEF in heart failure mice
10 minutes 7 days a week for 1 week
https://pmc.ncbi.nlm.nih.gov/articles/PMC8674466/#s2

** extremely low intensity effective over day , 10.1159/000335547
freely moving under 0.042 mw/cm2 red light at surface for 12 hrs a day
showed improved stroke recovery when given for 20 days after induction

10.1007/s00221-016-4578-8
LED held over mouse head for 90 seconds in parkinsons x2 daily 3 hrs apart, effects lasted for 2 days after use

We have estimated the energy levels reaching the midbrain at 5.3 mW/cm2 SO I guess was at least ~50 mw/cmw at head level

NIr was fast-acting and long-lasting; behavioural improvements were almost immediate following treatment and lasted for several days thereafter.

preventative effect, and faster recovery to baseline (IMMEDIATE RECOVERY FROM A TOXIN)

It should be noted that in the MPTP-NIr (post-treatment) group, the locomotor activity of mice returned to baseline almost immediately after the first NIr treatment on 7d; in fact, these animals were moving more freely around their box within only 20 min after their NIr treatment. The activity of the mice in the MPTP group by contrast was still much lower than baseline over the 2 days post-MPTP injections (45–55 %)
(mptp-nir is delayed until day 7)
*{one group was given nir at same time or before only, and the recovery effect was still there 2 days after}
*We showed that NIr treatment was able to “protect” cells against a toxic insult and limit motor deficits both when applied immediately (within minutes; i.e. simultaneous-treatment) or when applied up to 2 days beforehand (i.e. pre-treatment)

Just 5 minutes a week (20mw/cm2) increased lifespan, bone size, cartilage amount in aged mice
https://www.sciencedirect.com/science/article/pii/S1011134424001179

https://pmc.ncbi.nlm.nih.gov/articles/PMC8389381/#sec012

We show that Imidacloprid undermines bumblebee respiration and immunocompetence and that this can be rescued by 670nm exposures of 1min. This positive impact lasts for 4–6 days in both cases and implies that respiration and immunocompetence have common underlying mechanisms that likely resides with mitochondrial integrity.

0.5 mins exposure had no impact. In healthy bumblebees, 1 min exposure increased respiration significantly, but longer times did not result in a further increase (Fig 1B and 1C).
Elevated respiration from single exposures in healthy bees lasted 144h

1 minute of 40 mw/cm2 intensity

Co2 production higher than controls too

And its BEES so another in mammals https://pubmed.ncbi.nlm.nih.gov/26426815/

4 minutes a day stopped the increased immune cell killing of their endothelial cells in diabetic mice

Ray said just a couple minutes, or a quick flash, is enough to help stimulate mitochondria and quench excited electrons that are out of their proper flow

Just shining red light through their head. It restores their mitochondrial oxidation very quickly. - And then it'll continue on its own for a bit without the light? - Yeah, it has a certain momentum before the stress knocks it out again. So about every 10 or 15 minutes having a little flash of light on your body would probably help

Here the momentum lasted 2 hours in humans, stimulates glucose usage / co2 production from 15 mins
https://onlinelibrary.wiley.com/doi/10.1002/jbio.202300521

If you shine just a burst of red light it quenches excited electrons , puts electrons back in the proper arrangement

Within the first hour after exposing frogs to that same intensity of gamma rays, if they shined bright red light on them, they didn't get sick at all.

more detailed writeup https://cs3001.substack.com/p/typical-room-brightness-might-not

Chronic inescapable stress creates a supersensitivity to some compounds e.g through the acetylcholine receptor, and giving the stressed animals an agonist causes core temperature to drop a lot.

These researchers found 7000 lux at eye level for 6 hours a day almost fully abolished this effect of stress induced by a compound. They used a fluorescent bulb (typically mainly orange and green wavelengths without much red and blue, but the researchers said they used "full spectrum" for this).

300 lux light at eye level for 6 hours didnt offset the effects of stress at all (a typical room might be 200 - 500 lux at eye level) .
and 7000lux for 2 hours only didn't either

7000lux for 8 hours also helped in the swim stress test

Low level lighting can still allow for lower daytime melatonin, but it’s slower than 2000 lux+ which gives a big effect in 15 minutes at 2000 lux vs 150 lux. https://academic.oup.com/jcem/article-abstract/86/1/151/2841140

A cloudy day can be ~1000 lux apparently (which happens to be where effects are seen to max out on some measures).

so this might be the threshold needed in the day to help offset some effects of high stress, or possibly 500lux, if not the full 7000. But for this effect >2 hours and up to 6 hours is needed.

(thermic = hypothermic)

@cs3000 & page 58 of archive

unsaturated carbon chains in some compounds , oxidizing (adding oxygen or another electron acceptor) = carbon dioxide produced , hydrating (adding water) = glucose produced
{interesting how that fits with cancer cells going by these reactions, co2 not produced as low oxygen to add, cancer cells water logged / more free water so producing more glucose for glycolysis}

Page 58 & 59 (55 & 56 of archive) really interesting https://archive.org/details/TheChemistryOfNaturalImmunityWilliamKoch/page/n55/mode/2up

Kochs idea was the hyper-proliferation seen in cancer was an attempt at diluting energy disrupting substances so they can be more easily oxidized, from their distorted large polymer forms back to forms that can participate in cells functioning

"Some day when the multiplication is rapid enough compared with the production of the toxic factor the evolutionary purpose of the whole effort will be realized, and an efficient oxidizing machine will be produced which should serve as a protection not only against malignancy, but also against all of its associated allergic expressions, old age changes, and the lowered resistance against infection. "

@Mauritio its an aldehyde so doesnt sound like a healthy compound / pro metabolic outside of specific use nah (probably best not to take generally outside of cancer) so its one of those things that has an outweighing effect on cancer cells, the anti-cancer dose didnt show toxicity though so i guess healthy cells can deal with that amount well enough but probably some extra burden

apparently low amounts can stimulate cancer growth but higher amounts triggers the apoptosis process (thats normally lacking because of damage making it inefficient, and pH change, needs to be more acidic inside the tissue for apoptosis to happen optimally, cancers are acidic nearby but higher end pH inside - maybe methylglyoxal accumulation makes the tumor more acidic?)

Also it has two carbonyl groups , koch and reinstorff back in the day thought these can be used to take out things with strong bonds that are sticking around blocking mitochondria respiration and enable healthy processes again. maybe @dapose has more to to say on that

Following Glo1 inhibition, cancer cells switch from glycolysis to tricarboxylic acid (TCA) cycle to avoid apoptosis induced by MGO accumulation.

for some reason cancer cells are more sensitive to MGO overload than normal ones even though they have higher glutathione (which sounds to me like the effect is less about damage and more about re-enabling apoptosis unless for some reason they accumulate extreme amounts) https://www.science.org/doi/10.1126/science.160.3832.1140.a