The Johns 2002 paper goes into many depths:

"Specifically, ultrasound has been shown to modulate vasoconstriction; lymphocyte adhesion properties of endothelium, mast cell degranulation, phagocytosis by macrophage, production of growth factors by macrophages; calcium fluxes in fibroblasts; angiogenesis; proliferation of T-cells, osteoblasts, fibroblasts, and a number of proteins associated with inflammation and repair (IL-1, IL-2, IL-6, IL-8, interferon-γ, fibroblast growth factor-b, vascular endothelial growth factor, collagen) (Table)1,34,40–42,45–53; and to accelerate thrombolyisis.7–16 In general, most of these researchers used a frequency of 1 MHz or 3 MHz, and the intensities ranged from 0.1 to 1.5 W/cm2. An alternative therapeutic protocol employs a frequency of 45 kHz. An intensity range of 5 to 100 mW/cm2 was shown to increase the production of IL-1, IL-8, vascular endothelial growth factor, fibroblast growth factor-b, and collagen; promote bone healing; and accelerate thrombolysis.5,45,54,55 The long-wave (45-kHz) ultrasound increases penetration depth and, therefore, seems to be more appropriate than traditional high-frequency ultrasound (1 MHz and 3 MHz) for promoting revascularization and bone healing at greater depths."

increase in function

"The frequency resonance hypothesis suggests that the energy provided to the enzyme by the ultrasound wave may induce transient conformational shifts in certain enzymatic proteins, altering the enzyme's activity (ie, kinases or phosphatases) and the overall function of the cell (Figure 1). Alternatively, ultrasound's resonating force may result in the dissociation of functional multimolecular complexes (Figure 2) or the release of a sequestered molecule by dislodging an inhibitor molecule from the multimolecular complex (Figure 3). In essence, the mechanism of ultrasound's action in Figures 2 and 3 is the same. " "Hypothetically, frequency resonance may imply that different frequencies (1 MHz, 3 MHz, 45 kHz, and others) establish unique resonant or shearing forces (or both). Moreover, various frequencies may affect combinations of proteins or multimolecular complexes in different ways, lending to the possibility of targeted effects at the cellular and molecular levels."

@cs3000 well said 👌🏻

@Rah1woot right I didn’t know until last year that different things absorb in different mediums.

@alfredoolivas it appears you are correct on that one! Finally, real progesterone.

@hcwilliams said in How would you come off of TRT without PCT?:

Sounds good.

Been 5 days since last injection. E2 and serotonin notably still high.

I would also add some Aspirin (plus K2-mk4) to the mix, which should help lower serotonin and E2 levels. And also "refresh the pipes"... 😉

More about it: https://testonation.com/2023/05/22/aspirin-on-testosterone-dopamine-and-sexual-function/

@LetTheRedeemed you are right about hopelessness and it is definitely manifest in my life. It's something I have to deal with on a spiritual level. I've known about Peat for about seven years but never took a deep dive into all his thought until about a year and a half ago. I think the main thing that kept me away was the pro-sugar thing, but learning more about the thyroid and how pufa disupts sugar metabolism helped me warm up to it. I have candida and sugar makes it worse, even with flowers of sulfur, so that's part of why I didn't like it, even though I always trusted natural sugar in moderation over alternative sweeteners. It was lab-tested and confirmed in 2018 but I've had candida symptoms (such as thrush) since I was around 12-13 which was '09-10. I think it was caused by stress in childhood. The thrush is not just white tongue but on areas of my body, I can use clotrimazole and betamethasone dipropionate (a steroid cream) to manage it on occasion when needed. I think my gut is likely related to my low testosterone but I haven't solved that yet either despite my best efforts with natural remedies and even some medications like fluconazole (which did nothing discernible). As for thyroid I usually take daily one cynomel and one cynoplus, crush them within the folds of some parchment paper using a mortar and pestle and put the powder in a mug to spoon out from on the tip of a teaspoon, just eyeballing it in proportion to how much I'm eating with it. Besides raw carrots / endotoxin clearers and going for walks I don't recall what else works for gut motility.

@metabolicmilk

Still waiting on my shipment from PPL. Will update this thread after I dissolve the powder and again after a few weeks of using it.

@izkrov Yeah that's Ray's take on it. It's important to understand the TRT community has very different views, and actually isn't that compatible with ours. We do "patch work" here to mix some of the ideas in the TRT community with bioenergetics.

Standard TRT protocol is usually something like 120 up to 250 a week. Typically test cypionate. 4mgs won't raise blood levels which is what the TRT is purposed for.

Of course this can cause issues. For one, test cypionate is a longer acting ester. Your levels won't mimic the diurnal rhythm. I don't think we've yet fully understood the implications of this. Most of the "bros" seems to think this is good, because longer esters mean more stabilized androgens at a higher level.

But this could have serious adverse effects too. Like being at an increased risk of hyperestrogenicity, clots, stroke, and the like. It will have a prolonged suppression on the HPTA as well, lowering fertility (unless you take hCG, which has it's own set of risks) and eventually upstream hormones like P5, P4, and DHEA.

@SexyPizza I think just PG and VitE would work for most things. Maybe if your vitamin E oil is too thick you could add some oleic acid to thin it out, but other then that you should be good.

Or you could try oleic acid (OA) + PG + Limonene. But I generally don't see the need of adding two lipid-based solvents together. That's just my 2 cents, I'm very far from an expert in this regard. @brightside seems far more knowledgeable in this area. He seems to be well versed in making emulsions, which would enhance the penetration of lipid-based solutions even more than free base.

Which ever way you decide, these solutions will be extremely "nonvolatile." So you're looking at something that will have a more slow, but continuous, absorption rate. Which I think is probably good, especially for people who only want to dose one time per day. The only downside is it will take a while to dry on skin.

So I would give application site at least 30 minutes of air time to dry before putting on a shirt or pants. And if you're pressed for time and can't wait that long, you could always gently wrap the application site in plastic wrap before putting clothes on over it.

@b1 said in Effect of Aspirin on Male Hormones, Testicular and Epididymal Histology In Mice:

@GreekDemiGod
So take iron supplements or consume more iron to counteract this?

I personally wouldn't. Iron supplements are dangerous, and it can be a potent oxidizer. Almost as bad as PUFA.

Unless you've got cancer, there's never really a reason to take more than 1,000mg a day. Even less in most cases will work. 500mg is enough to lower stress and free fatty acids.

@alfredoolivas

Thank you, not sure if I notice any different anyway, been doing transdermal T with different formulas in a while.

Now doing forearms and back of knees. I was doing shoulders but I think alcohol irritated the skin there so I changed to forearms.

I just got DMSO so this is the first batch with DMSO. We'll see how it goes

@oldchem Makes so much sense, thank you! Also worth mentioning since my brain injury I put on about 20lbs in the past 2 years, so have more bodyfat than before so this is all starting to make sense.

@ATP do you use BCAA?